PMID: 8944628Nov 1, 1996Paper

ATP activates K and Cl channels via purinoceptor-mediated release of Ca2+ in human coronary artery smooth muscle

The American Journal of Physiology
D StrøbaekS P Olesen

Abstract

Coronary artery smooth muscle cells express G protein-coupled purinoceptors, and we report here for the first time how receptor activation by extracellular ATP influences cell membrane currents and membrane potential in human cells. ATP (100 microM) stimulated a triphasic change in membrane potential lasting several seconds, which was caused by sequential opening of transient inward and outward conductances. The inward current was carried by Cl- and the outward current by K+, as shown by ion substitution and changes in holding potential. Both currents were independent of the presence of external Ca2+ but were blocked by strong buffering of Ca2+ in the internal solution. The P2u- and P2y-purinoceptor agonists UTP and 2-methylthioadenosine 5'-triphosphate activated similar currents, whereas the P2x-receptor agonist alpha, beta-methyleneadenosine 5'-triphosphate and the P1-receptor agonist adenosine failed to stimulate any whole cell currents. The ATP-activated K+ current was inhibited by iberiotoxin (200 nM), and it was potentiated by the BK channel activator NS-1619 (30 microM). In cell-attached recordings, ATP activated a 230-pS BK channel. In conclusion, ATP acting via P2 purinoceptors stimulated release of Ca2+ from internal ...Continue Reading

References

Mar 1, 1992·Trends in Pharmacological Sciences·B P Bean
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Citations

Mar 10, 1999·The American Journal of Physiology·E W InschoJ D Imig
May 18, 2001·American Journal of Physiology. Heart and Circulatory Physiology·D G Welsh, J E Brayden
Feb 13, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Nari KimEuiyong Kim
May 12, 2018·Pflügers Archiv : European journal of physiology·Alex M DopicoJonathan H Jaggar
Nov 22, 2000·American Journal of Physiology. Heart and Circulatory Physiology·P J WhiteM R Boarder
Jan 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H Koegel, C Alzheimer
Apr 21, 2010·Experimental Biology and Medicine·Gregory M Dick, Johnathan D Tune
Dec 15, 2000·Acta Physiologica Scandinavica·T Hafting, O Sand
Nov 1, 2005·British Journal of Pharmacology·Karima SerirRostislav Bychkov
Feb 15, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Laszlo KölesPeter Illes
Feb 22, 2000·FEBS Letters·E MauryH Chap
Dec 18, 2004·American Journal of Physiology. Heart and Circulatory Physiology·S A ReadingJ E Brayden
Aug 15, 2000·Journal of Cellular Physiology·K Lange
Aug 18, 2012·Journal of Cell Science·D MacMillanJ G McCarron
Oct 3, 2000·Journal of Theoretical Biology·K Lange
Aug 22, 2000·Journal of Hepatology·A P FeranchakR M Roman
May 19, 1998·Trends in Pharmacological Sciences·M R Boarder, S M Hourani
Nov 14, 1998·Biochimica Et Biophysica Acta·S F PedersenE K Hoffmann
Jan 16, 1999·Biochimica Et Biophysica Acta·S PedersenE K Hoffmann

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