PMID: 8969904Nov 1, 1996Paper

ATP and a mitochondrial electrochemical gradient are required for functional activity of the steroidogenic acute regulatory (StAR) protein in isolated mitochondria

Endocrine Research
S King, D M Stocco

Abstract

The Steroidogenic Acute Regulatory (StAR) protein has been put forth as the rapidly synthesized, cycloheximide-sensitive protein that is required for the transport of cholesterol to the inner mitochondrial membrane and the P450scc enzyme and thereby acutely regulates steroidogenesis in steroidogenic tissues. In this study, several of the factors that may be required for StAR activity were examined using an in vitro system. Lysates from StAR-transfected COS-1 cells were added to mitochondria isolated from MA-10 Leydig tumor cells. Results obtained demonstrated that StAR-containing cell lysate increased steroidogenesis in isolated mitochondria, but failed to do so in the presence of m-CCCP, apyrase, or AMP-PNP, suggesting that StAR function requires ATP hydrolysis as well as an electrochemical gradient for maximal steroidogenic activity.

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Citations

Jan 16, 2016·Molecular Endocrinology·Ye LinFredric B Kraemer
Aug 26, 2014·Cytoskeleton·Sobia Iqbal, David A Hood
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·H S BoseW L Miller
Apr 13, 2007·Cell Biology and Toxicology·S L LevineV Papadopoulos
Feb 14, 2006·Annals of the New York Academy of Sciences·Dale B HalesKaren Held Hales
Sep 21, 2000·Molecular and Cellular Endocrinology·S R KingD M Stocco
May 30, 2001·Advanced Drug Delivery Reviews·I E Scheffler
May 30, 2006·Biochimica Et Biophysica Acta·Erica Chevalier-Larsen, Erika L F Holzbaur
Jan 16, 2007·Molecular and Cellular Endocrinology·Zvi GranotJoseph Orly
Jul 17, 2007·Neuropharmacology·Angela Maria FalchiGiacomo Diaz

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