ATP binding by the P-loop NTPase OsYchF1 (an unconventional G protein) contributes to biotic but not abiotic stress responses

Proceedings of the National Academy of Sciences of the United States of America
Ming-Yan CheungHon-Ming Lam

Abstract

G proteins are involved in almost all aspects of the cellular regulatory pathways through their ability to bind and hydrolyze GTP. The YchF subfamily, interestingly, possesses the unique ability to bind both ATP and GTP, and is possibly an ancestral form of G proteins based on phylogenetic studies and is present in all kingdoms of life. However, the biological significance of such a relaxed ligand specificity has long eluded researchers. Here, we have elucidated the different conformational changes caused by the binding of a YchF homolog in rice (OsYchF1) to ATP versus GTP by X-ray crystallography. Furthermore, by comparing the 3D relationships of the ligand position and the various amino acid residues at the binding sites in the crystal structures of the apo-bound and ligand-bound versions, a mechanism for the protein's ability to bind both ligands is revealed. Mutation of the noncanonical G4 motif of the OsYchF1 to the canonical sequence for GTP specificity precludes the binding/hydrolysis of ATP and prevents OsYchF1 from functioning as a negative regulator of plant-defense responses, while retaining its ability to bind/hydrolyze GTP and its function as a negative regulator of abiotic stress responses, demonstrating the speci...Continue Reading

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Citations

Feb 24, 2017·Journal of Inorganic Biochemistry·Shailender Kumar VermaYusuf Akhter
Jul 25, 2017·International Journal of Molecular Sciences·Lara Esch, Ulrich Schaffrath
Feb 12, 2019·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Nirujah BalasingamNehal Thakor
Feb 15, 2019·Proceedings of the National Academy of Sciences of the United States of America·Minhye ShinShelley M Payne
Feb 23, 2017·The Journal of Biological Chemistry·Sotirios GkekasWim Versées
May 1, 2019·International Journal of Molecular Sciences·Qing DongXi-Hong Shen
Oct 20, 2018·International Journal of Molecular Sciences·Yee-Shan KuHon-Ming Lam
Sep 22, 2021·Bioinformatics·Liang RaoGui-Jun Zhang

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