ATP-binding cassette transporters in immortalised human brain microvascular endothelial cells in normal and hypoxic conditions.

Experimental & Translational Stroke Medicine
Christian LindnerFelix Schlachetzki

Abstract

Rapid reperfusion following ischemia is the most effective therapy in stroke therapy. However, the success may be compromised by ischemia & reperfusion (I/R) injury and at the human blood-brain barrier (BBB), therefore the effects on transendothelial transport are of special interest. Current studies suggest the ATP-binding cassette (ABC) transporters to be regulated upon ischemic stroke in a way that impedes the effects of drug therapy. The immortalised human brain microvascular endothelial cell line hCMEC/D3 provides most of the unique properties of the BBB with respect to transport and might be a reliable in vitro model to study transendothelial transport after I/R. We exposed hCMEC/D3 cells to 24 hours of hypoxia alone and to hypoxia followed by 60 min of reoxygenisation as an in vitro model for I/R. Western blot showed mild upregulation of hypoxia inducible factor (HIF-1α) after hypoxia alone and RNA lysates were analysed with a well-established real-time RT-PCR-based TaqMan low-density array detecting 47 of 48 known human ABC transporters. No significant increases of ABC mRNA expression levels were detected neither in hypoxic nor in I/R samples. However, slight decrease of ABCC1 in hypoxic and I/R samples and of ABCA10 an...Continue Reading

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Citations

Feb 13, 2016·Journal of Pharmaceutical Sciences·Erica Tornabene, Birger Brodin
Apr 19, 2016·Brain Research·Nurul Adhwa RahmanSiti Hanna Muharram
Jul 8, 2016·Journal of Clinical Pharmacology·Amandla Atilano-RoqueMelanie S Joy
Oct 18, 2016·American Journal of Human Genetics·Sirui ZhouGuy A Rouleau
Nov 25, 2017·Journal of Alzheimer's Disease : JAD·Cátia D PereiraSandra Rebelo
Dec 31, 2020·International Journal of Molecular Sciences·Alexy Tran-DinhOlivier Meilhac

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