ATP binding induces large conformational changes in the apical and equatorial domains of the eukaryotic chaperonin containing TCP-1 complex.

The Journal of Biological Chemistry
O LlorcaJ M Valpuesta

Abstract

The chaperonin-containing TCP-1 complex (CCT) is a heteromeric particle composed of eight different subunits arranged in two back-to-back 8-fold pseudo-symmetric rings. The structural and functional implications of nucleotide binding to the CCT complex was addressed by electron microscopy and image processing. Whereas ADP binding to CCT does not reveal major conformational differences when compared with nucleotide-free CCT, ATP binding induces large conformational changes in the apical and equatorial domains, shifting the latter domains up to 40 degrees (with respect to the inter-ring plane) compared with 10 degrees for nucleotide-free CCT or ADP-CCT. This equatorial ATP-induced shift has no counterpart in GroEL, its prokaryotic homologue, which suggests differences in the folding mechanism for CCT.

References

Jan 1, 1992·Ultramicroscopy·P PenczekJ Frank
Jan 1, 1991·Annual Review of Biochemistry·R J Ellis, S M van der Vies
Jan 1, 1987·Ultramicroscopy·M UnserA C Steven
Dec 1, 1995·The Journal of Biological Chemistry·E Quaite-RandallA Joachimiak
Feb 1, 1995·European Journal of Biochemistry·T WaldmannW Baumeister
May 30, 1994·FEBS Letters·O LlorcaJ M Valpuesta
Jan 26, 1996·Journal of Molecular Biology·O Yifrach, A Horovitz
Oct 1, 1995·Molecular Microbiology·J A Baum, T Malvar
Oct 1, 1996·Journal of Structural Biology·R MarabiniJ M Carazo
Feb 1, 1997·Journal of Structural Biology·O LlorcaJ M Valpuesta
Apr 1, 1997·Protein Science : a Publication of the Protein Society·W A Fenton, A L Horwich
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·P Lin, F Sherman
May 1, 1993·Current Biology : CB·H R SaibilR J Ellis

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Citations

Jul 20, 2000·Micron : the International Research and Review Journal for Microscopy·J L CarrascosaJ M Valpuesta
Feb 8, 2005·Nature Structural & Molecular Biology·Dalia Rivenzon-SegalAmnon Horovitz
Dec 14, 2001·Biochemical and Biophysical Research Communications·R IizukaM Yohda
May 5, 2006·Protein Science : a Publication of the Protein Society·Erik J MillerJudith Frydman
Oct 15, 2013·Journal of Molecular Biology·Ayumi NakagawaMasafumi Yohda
Apr 11, 2008·Structure·Daniel K ClareHelen R Saibil
Oct 3, 2001·Journal of Structural Biology·A HorovitzO Yifrach
Mar 15, 2000·Journal of Molecular Biology·G SchoehnH R Saibil
Nov 10, 2000·Journal of Molecular Biology·M Ritco-Vonsovici, K R Willison
Apr 9, 2008·Archives of Biochemistry and Biophysics·Maria Giulia Bigotti, Anthony R Clarke
May 30, 2007·The Journal of Biological Chemistry·Masak Takaine, Issei Mabuchi
Oct 2, 2002·Plant & Cell Physiology·Akiyo YamadaYoshihiro Ozeki
Apr 5, 2007·Molecular Biology and Evolution·Valentin Ruano-Rubio, Mario A Fares
Dec 10, 1999·Microbiology and Molecular Biology Reviews : MMBR·A J MacarioE Conway de Macario

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