ATP-binding to P-type ATPases as revealed by biochemical, spectroscopic, and crystallographic experiments

Proteins
Martin Kubala

Abstract

P-type ATPases form a large family of cation translocating ATPases. Recent progress in crystallography yielded several high-resolution structures of Ca(2+)-ATPase from sarco(endo)plasmic reticulum (SERCA) in various conformations. They could elucidate the conformational changes of the enzyme, which are necessary for the translocation of cations, or the mechanism that explains how the nucleotide binding is coupled to the cation transport. However, crystals of proteins are usually obtained only under conditions that significantly differ from the physiological ones and with ligands that are incompatible with the enzyme function, and both of these factors can inevitably influence the enzyme structure. Biochemical (such as mutagenesis, cleavage, and labeling) or spectroscopic experiments can yield only limited structural information, but this information could be considered relevant, because measurement can be performed under physiological conditions and with true ligands. However, interpretation of some biochemical or spectroscopic data could be difficult without precise knowledge of the structure. Thus, only a combination of both these approaches can extract the relevant information and identify artifacts. Briefly, there is good a...Continue Reading

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Citations

Mar 7, 2008·Pharmacological Reviews·Daniel J MüllerKrzysztof Palczewski
Nov 5, 2015·Journal of the American Society for Mass Spectrometry·M Ariel Geer, Michael C Fitzgerald
Aug 21, 2007·Archives of Biochemistry and Biophysics·Lenka GrycovaJan Teisinger
May 14, 2011·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Francisco A LeoneIvana A Borin
Apr 12, 2016·Frontiers in Physiology·Martin KubalaDavid Biedermann
Feb 24, 2009·Biochimica Et Biophysica Acta·Lenka GrycovaMartin Kubala
Aug 5, 2010·Physical Chemistry Chemical Physics : PCCP·Marika JanovskáJitka Ulrichová

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