PMID: 7372713May 1, 1980Paper

ATP dependence of H+ secretion

The Journal of Cell Biology
T BerglindhG Sachs

Abstract

Cells in isolated rabbit gastric gland were made permeable to ATP by high voltage discharge across a gland suspension. In both normal (5.4 mM K+) and high K+ (108 mM) medium, this electrical shock resulted in a marked reduction in the ability of the parietal cell to produce and accumulate acid. Acid production was monitored both microscopically by acridine orange accumulation in the secretory canaliculus and by accumulation of the weak base [14C]aminopyrine. In 108 mM K+ solutions but not in 5.4 mM K+ solutions 5, mM ATP was able to restore the accumulation of these probes to control (unshocked) levels. When shocked glands had been previously stimulated by secretagogues, the aminopyrine accumulation ratio was only partly restored by ATP. Inhibition of mitochondrial respiration by cyanide, azide, or Amytal abolished acid secretion; the subsequent addition of ATP to shocked glands increased the aminopyrine accumulation ratio to 47 and resulted in an acridine orange fluorescence indistinguishable from that of histamine-stimulated, unshocked glands. We conclude that ATP can act as a substrate for H+ secretion in the parietal cell, and that perhaps no additional energy source is necessary.

References

Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D R DiBonaG Sachs
Mar 8, 1979·Biochimica Et Biophysica Acta·E RabonG Sachs
Jul 14, 1977·The Journal of Membrane Biology·E C RabonG Sachs
Feb 1, 1976·Acta Physiologica Scandinavica·T Berglindh, K J Obrink
Sep 23, 1974·Biochemical and Biophysical Research Communications·J LeeP Scholes
Sep 16, 1974·Biochimica Et Biophysica Acta·S J Hersey
Jan 1, 1967·Biochimica Et Biophysica Acta·G SachsB I Hirschowitz
Apr 25, 1973·Biochimica Et Biophysica Acta·A L Ganser, J G Forte
Oct 29, 1974·Biochimica Et Biophysica Acta·R P DurbinA Nickel
Aug 20, 1968·Biochimica Et Biophysica Acta·G SachsB I Hirschowitz

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Citations

Jan 1, 1986·Histochemistry·K FujimotoK Ogawa
Jan 1, 1983·The Journal of Membrane Biology·J M Diamond, T E Machen
Jan 1, 1982·The Journal of Membrane Biology·G SachsE Rabon
Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·G SachsS J Hersey
Jan 1, 1989·Comparative Biochemistry and Physiology. A, Comparative Physiology·J A ArrudaK Thomasson
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D H MalinowskaG Sachs
Oct 1, 1986·Calcified Tissue International·R E AndersonW S Jee
Dec 1, 1985·Calcified Tissue International·R E AndersonD M Woodbury
Jan 1, 1985·Scandinavian Journal of Gastroenterology. Supplement·T Berglindh, G Sachs
Dec 1, 1981·The Journal of Surgical Research·T K Ray, D Fromm
Jan 1, 1982·Annals of the New York Academy of Sciences·L FallerA Smolka
Jan 1, 1980·Annals of the New York Academy of Sciences·G SachsG Saccomani
Jan 1, 1989·Drug Metabolism and Drug Interactions·G Saccomani, S J Hersey
May 1, 1991·The American Journal of Physiology·G SaccomaniR L Shoemaker

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