PMID: 2492420Feb 1, 1989Paper

ATP-dependent Ca2+ transport in the rat parotid basolateral plasma membrane is regulated by calmodulin

Archives of Biochemistry and Biophysics
I S AmbudkarB J Baum

Abstract

Calmodulin regulation of ATP-dependent Ca2+ transport activity was assessed in inverted basolateral plasma membrane vesicles (BLMV) isolated from rat parotid glands. The initial rate of Ca2+ transport in media containing 100 nM Ca2+ was stimulated by approximately 60% at maximal concentrations (300 nM) of exogenously added calmodulin (CAM). Half-maximal activation was obtained at 50 and 175 nM CAM in KCl and mannitol containing assay media, respectively. In the KCl medium, addition of 300 nM CAM increased the affinity of the BLMV Ca2+ transport activity for Ca2+ from approximately 70 nM, in the absence of added CAM, to approximately 50 nM. Vmax was consistently increased by approximately 20% under these conditions. When BLMV were treated with ethylene glycol bis(beta-aminoethylether) N,N'-tetraacetic acid (EGTA) (200 microM), the affinity of the transporter for Ca2+ decreased by 50% to approximately 150 nM, with no change in Vmax. When CAM was added to the EGTA-treated membranes, Ca2+ transport activity was comparable to that obtained when CAM was added directly to control, untreated BLMV. The CAM antagonists, trifluoperazine (TFP), W-7, and calmidazolium, inhibited Ca2+ transport in the presence of CAM. Half-maximal inhibition...Continue Reading

References

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Citations

Apr 16, 2009·Cell Biochemistry and Function·José NicolauMarcela Carrilho
Feb 20, 2007·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Fanasy P DemingFrancisco Rivera-Hidalgo
Feb 22, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·I S Ambudkar
Nov 8, 2017·Journal of Biophotonics·Juliana R de CastroAlyne Simões
Nov 26, 2002·The Plant Journal : for Cell and Molecular Biology·Frédéric D DuvalDavid Macherel
Nov 9, 2000·The Plant Journal : for Cell and Molecular Biology·W A Snedden, E Blumwald

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