ATP-dependent ferric hydroxamate transport system in Escherichia coli: periplasmic FhuD interacts with a periplasmic and with a transmembrane/cytoplasmic region of the integral membrane protein FhuB, as revealed by competitive peptide mapping

Molecular Microbiology
A MademidisV Braun

Abstract

The Escherichia coli iron transport system via ferrichrome belongs to the group of ATP-dependent transporters that are widely distributed in prokaryotes and eukaryotes. Transport across the cytoplasmic membrane is mediated by three proteins: FhuD in the periplasm, FhuB in the cytoplasmic membrane and FhuC (ATPase) associated with the inside of the cytoplasmic membrane. Interaction of FhuD with FhuB was studied in vitro with biotinylated synthetic 10 residue and 20-24 residue peptides of FhuB by determining the activity of beta-galactosidase linked to the peptides via streptavidin. Peptides identical in sequence to only one of the four periplasmic loops (loop 2), predicted by a transmembrane model of FhuB, and peptides representing a transmembrane segment and part of the adjacent cytoplasmic loop 7 of FhuB bound to FhuD. Decapeptides were transferred into the periplasm of cells through a FhuA deletion derivative that forms permanently open channels three times as large as the porins in the outer membrane. FhuB peptides that bound to FhuD inhibited ferrichrome transport, while peptides that did not bind to FhuD did not affect transport. These data led us to propose that the periplasmic FhuD interacts with a transmembrane region a...Continue Reading

Citations

Jan 1, 1991·Biology of Metals·V BraunK Hantke
Apr 27, 1999·Trends in Biochemical Sciences·V Braun, H Killmann
Jun 4, 2002·DNA and Cell Biology·Igor Stojiljkovic, Donna Perkins-Balding
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Jeremy J GilbreathDavid R Hendrixson
Dec 12, 2002·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth L BorthsDouglas C Rees
Aug 8, 2006·Molecular Microbiology·Marcus MiethkeMohamed A Marahiel
Feb 24, 2015·Immunological Reviews·Olivier NeyrollesMichael Niederweis
Jun 28, 2003·FEMS Microbiology Reviews·Simon C AndrewsFrancisco Rodríguez-Quiñones
Sep 22, 2011·Biochimica Et Biophysica Acta·Claire J Parker SiburtAlvin L Crumbliss
Jan 14, 2004·Cell·Andrew D Ferguson, Johann Deisenhofer
Oct 6, 2000·Annual Review of Microbiology·C Ratledge, L G Dover
Sep 2, 1998·Molecular Microbiology·M EhrmannA Schlösser
Nov 13, 2002·Microbiology·Helmut KillmannVolkmar Braun
Aug 25, 2006·The Journal of Biological Chemistry·David M CarterJames W Coulton

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