PMID: 8584407Nov 1, 1995Paper

ATP-Dependent inhibition of Ca2+-activated K+ channels in vascular smooth muscle cells by neuropeptide Y

Pflügers Archiv : European journal of physiology
Z Xiong, D W Cheung

Abstract

Neuropeptide Y(NPY) inhibits Ca2+-activated K+ channels reversibly in vascular smooth muscle cells from the rat tail artery. NPY (200 microM) had no effect in the absence of intracellular adenosine 5'-triphosphate (ATP) and when the metabolic poison cyanide-M-chlorophenyl hydrozone (10 microM) was included in the intracellular pipette solution. NPY was also not effective when ATP was substituted by the non-hydrolysable ATP analogue adenosine 5'-[beta gamma-methylene]-triphosphate (AMP-PCP). NPY inhibited Ca2+-activated K+ channel activity when ATP was replaced by adenosine 5'-O-(3-thiotriphosphate) (ATP [gamma-S]) and the inhibition was not readily reversed upon washing. Protein kinase inhibitor (1 microM), a specific inhibitor of adenosine 3', 5'-cyclic monophosphate-dependent protein kinase, had no significant effect on the inhibitory action of NPY. The effect of NPY on single-channel activity was inhibited by the tyrosine kinase inhibitor genistein (10 microM) but not by daidzein, an inactive analogue of genistein. These observations suggest that the inhibition by NPY of Ca2+-activated K+ channels is mediated by ATP-dependent phosphorylation. The inhibitory effect of NPY was antagonized by the tyrosine kinase inhibitor genis...Continue Reading

References

Jan 14, 1992·European Journal of Pharmacology·D L SmallD W Cheung
Dec 30, 1992·Biochemical and Biophysical Research Communications·S WijetungeA D Hughes
Dec 1, 1988·The American Journal of Physiology·R J McAnultyG J Laurent
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Mar 1, 1994·Current Biology : CB·S A Siegelbaum
Dec 1, 1994·Pflügers Archiv : European journal of physiology·Z Xiong, D W Cheung
Dec 1, 1993·Pflügers Archiv : European journal of physiology·B J BolzonD W Cheung
Jun 1, 1993·Pflügers Archiv : European journal of physiology·Z XiongD W Cheung

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Citations

Apr 6, 2012·Current Osteoporosis Reports·Ee Cheng Khor, Paul Baldock
Nov 6, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Antonio P SilvaEric Grouzmann
Sep 15, 2009·Neuropeptides·Nicola J Lee, Herbert Herzog
Dec 1, 1998·The Journal of Physiology·T TakeuchiT Takewaki
Nov 5, 2013·Progress in Neurobiology·Ana Santos-CarvalhoCláudia Cavadas
Sep 5, 2002·Biopolymers·A Bettio, A G Beck-Sickinger

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