ATP-dependent leader peptide cleavage by NukT, a bifunctional ABC transporter, during lantibiotic biosynthesis

Journal of Bioscience and Bioengineering
Mami NishieKenji Sonomoto

Abstract

NukT, a possible ABC transporter maturation and secretion (AMS) protein, may contribute to the cleavage of the leader peptide of NukA, which is the prepeptide of the lantibiotic nukacin ISK-1, and to nukacin ISK-1 transport. In this study, we reconstituted in vitro peptidase activity of the full-length NukT overexpressed in inside-out membrane vesicles of Staphylococcus carnosus TM300. We found that the presence of unusual amino acids in NukA is required for leader peptide cleavage. Furthermore, NukT peptidase activity was inhibited by phenylmethylsulfonyl fluoride, a serine/cysteine protease inhibitor; this finding strongly suggests that NukT, like other AMS proteins, is a cysteine protease. Interestingly, NukT peptidase activity depended on ATP hydrolysis. These results suggest that the N-terminal peptidase domain of NukT may cooperatively function with the C-terminal ATP-binding domain. This is the first in vitro study on lantibiotics that reports the processing mechanism of a full-length bifunctional ABC transporter.

References

Jun 15, 1995·European Journal of Biochemistry·H G SahlG Bierbaum
Jun 1, 1997·Bioscience, Biotechnology, and Biochemistry·H KimuraA Ishizaki
Feb 3, 1999·Annals of the New York Academy of Sciences·H KimuraA Ishizaki
May 12, 2001·FEMS Microbiology Reviews·O McAuliffeC Hill
Jul 5, 2003·The Journal of Biological Chemistry·Galya ReuterHendrik W van Veen
Jul 26, 2005·Bioscience, Biotechnology, and Biochemistry·Jun-ichi NagaoKenji Sonomoto
Sep 7, 2005·Biochemical and Biophysical Research Communications·Jun-Ichi NagaoKenji Sonomoto
Nov 14, 2006·FEMS Microbiology Reviews·Alain DufourJean-Paul Le Pennec
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Amy L DavidsonJue Chen
Jun 24, 2008·Biochemistry·L A Furgerson IhnkenWilfred A van der Donk

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Citations

Apr 25, 2012·Applied Microbiology and Biotechnology·Steven BoakesJesús Cortés
Feb 10, 2011·The Journal of Biological Chemistry·Mami NishieKenji Sonomoto
Mar 13, 2012·Annual Review of Biochemistry·Patrick J Knerr, Wilfred A van der Donk
Aug 30, 2014·FEMS Microbiology Reviews·Joseph J GillespieAbdu F Azad
Jan 31, 2017·Chemical Reviews·Lindsay M RepkaWilfred A van der Donk
Nov 30, 2018·Journal of Industrial Microbiology & Biotechnology·Weixin TangWilfred A van der Donk
Apr 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Dee Dee LuuPamela C Ronald
Mar 22, 2018·Applied Microbiology and Biotechnology·Sen Zheng, Kenji Sonomoto
Apr 10, 2019·Microbiology·Emmanuele Severi, Gavin H Thomas
Oct 12, 2020·FEBS Letters·Sander H J SmitsKonstantinos Beis
Sep 29, 2019·Current Opinion in Genetics & Development·Benjamin J WillsonGavin H Thomas
Aug 12, 2019·Research in Microbiology·Konstantinos Beis, Sylvie Rebuffat
May 9, 2013·Journal of the American Chemical Society·Ginka H VöllerRoderich D Süssmuth
Dec 6, 2021·Protein Science : a Publication of the Protein Society·Shibani Bhattacharya, Anthony Palillo

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