ATP depletion affects the phosphorylation state, ligand binding, and nuclear transport of the 4 S polycyclic aromatic hydrocarbon-binding protein in rat hepatoma cells.

The Journal of Biological Chemistry
R BhatE Bresnick

Abstract

In the rat, cytochrome P4501A1 gene expression is thought to be regulated by several trans-acting factors including the 4 S polycyclic aromatic hydrocarbon (PAH)-binding protein. Phosphorylation and dephosphorylation have been suggested to influence the function of many cytosolic receptors and transcription factors. The ATP level within H4IIE rat hepatoma cells could be depleted by treatment with sodium azide or 2,4-dinitrophenol; restoration of the original ATP levels occurred with addition of glucose to the cell culture. ATP depletion reduced the phosphate content of the 4 S protein by approximately 25-30%, which lowered the binding of benzo[a]pyrene (B[a]P) to the 4 S protein by >60%. This effect could not be reversed by the addition of ATP to the binding reaction mixtures. Alkaline phosphatase treatment of the purified 4 S protein in a cell-free system also reduced the B[a]P binding to the protein. Cells treated with a protein phosphatase inhibitor, okadaic acid, and a protein kinase inhibitor, staurosporin, affected the B[a]P binding of the 4 S protein positively and negatively, respectively. These data suggested that phosphorylation is involved in the interaction of the 4 S protein with the PAH. The nuclear translocation ...Continue Reading

References

Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C J NielsenW B Pratt
Mar 1, 1992·Molecular and Cellular Biology·R H ChenJ Blenis
Aug 7, 1992·Cell·T Hunter, M Karin
Mar 7, 1991·Biochimica Et Biophysica Acta·J Garcia-BustosM N Hall
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W E Taylor, E T Young
Sep 1, 1990·Molecular and Cellular Biology·L M MylinJ E Hopper
Aug 31, 1990·Science·J H HurleyR M Stroud
Nov 15, 1987·Archives of Biochemistry and Biophysics·W H HouserE Bresnick
Oct 20, 1988·Nature·M Ptashne
Nov 15, 1986·Archives of Biochemistry and Biophysics·W H HouserE Bresnick
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R J Cook, C Wagner
Sep 1, 1983·Archives of Biochemistry and Biophysics·B TierneyE Bresnick
Apr 1, 1980·Archives of Biochemistry and Biophysics·B TierneyE Bresnick
Oct 1, 1995·Archives of Biochemistry and Biophysics·A RahaE Bresnick
Apr 1, 1992·Trends in Cell Biology·S P Jackson

❮ Previous
Next ❯

Citations

Jun 2, 2009·The Journal of Biological Chemistry·Zigmund LukaConrad Wagner
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·J C PolitzT Pederson
Sep 9, 2004·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Masumi KobayashiYukisato Ishida
Nov 14, 2012·Toxicology and Applied Pharmacology·Chia-Hung YenYi-Ming Arthur Chen
Jan 20, 2006·Pigment Cell Research·M J HoogduijnP G Genever
Mar 20, 1999·The Journal of Investigative Dermatology·P G GeneverT M Skerry
Feb 24, 2007·Biochemical and Biophysical Research Communications·Yoshinori SakataSeiichiroh Ohsako
Feb 17, 2017·International Journal of Cancer. Journal International Du Cancer·Chung-Hsien LiYi-Ming Arthur Chen
Mar 30, 2017·The Journal of Biological Chemistry·Sheng LinKenneth A Christensen
Aug 13, 2011·Journal of Neurosurgical Anesthesiology·Alexander ZlotnikVivian I Teichberg
Jan 24, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Martin KöhlerPer-Olof Berggren
Oct 27, 1997·The Journal of Biological Chemistry·N I Krupenko, C Wagner

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.