ATP-Driven Separation of Liquid Phase Condensates in Bacteria

Molecular Cell
Baptiste GuilhasMarcelo Nollmann

Abstract

Liquid-liquid phase-separated (LLPS) states are key to compartmentalizing components in the absence of membranes; however, it is unclear whether LLPS condensates are actively and specifically organized in the subcellular space and by which mechanisms. Here, we address this question by focusing on the ParABS DNA segregation system, composed of a centromeric-like sequence (parS), a DNA-binding protein (ParB), and a motor (ParA). We show that parS and ParB associate to form nanometer-sized, round condensates. ParB molecules diffuse rapidly within the nucleoid volume but display confined motions when trapped inside ParB condensates. Single ParB molecules are able to rapidly diffuse between different condensates, and nucleation is strongly favored by parS. Notably, the ParA motor is required to prevent the fusion of ParB condensates. These results describe a novel active mechanism that splits, segregates, and localizes non-canonical LLPS condensates in the subcellular space.

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Citations

Oct 31, 2020·Current Biology : CB·Phillip NußbaumSonja-Verena Albers
Nov 14, 2020·Biophysical Journal·Christopher A AzaldeguiJulie S Biteen
Jan 17, 2021·Biophysical Journal·Ibraheem AlshareedahPriya R Banerjee
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Jun 2, 2021·EcoSal Plus·Maxime LerouxRodrigo Reyes-Lamothe
Jun 25, 2021·Current Opinion in Microbiology·Gaurav Kumar, Sharmistha Sinha
Jul 14, 2021·Current Biology : CB·Jovana KaljevićGéraldine Laloux
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Aug 17, 2021·ELife·Adam Sb JalalTung Bk Le
Aug 17, 2021·Current Opinion in Cell Biology·Adrián Merino-SalomónPetra Schwille
Dec 22, 2020·ACS Nano·Anthony Birnie, Cees Dekker
Aug 4, 2021·Annual Review of Microbiology·Virginia S LioyFrédéric Boccard

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