Abstract
Eosinophils recognize various stimuli, such as cytokines, chemokines, immunoglobulins, complement, and external pathogens, resulting in their accumulation in mucosal tissues and the progression of inflammation. Eosinophils are also involved in innate Th2-type immune responses mediated through endogenous danger signals, including IL-33, uric acid (UA), or ATP, in non-sensitized mice exposed to environmental allergens. However, the mechanism involved in eosinophil responses to these danger signals remains insufficiently understood. We examined migration, adhesion, superoxide production and degranulation of human eosinophils. Isolated eosinophils were incubated with monosodium urate (MSU) crystals and ATPγS, a non-hydrolysable ATP analogue. To determine the involvement of P2 or P2Y2 receptors in eosinophil responses to UA and ATP, eosinophils were preincubated with a pan-P2 receptor inhibitor, oxidized ATP (oATP), or anti-P2Y2 antibody before incubation with MSU crystals or ATPγS. MSU crystals induced adhesion of eosinophils to recombinant human (rh)-ICAM-1 and induced production of superoxide. oATP abolished eosinophil responses to MSU crystals, suggesting involvement of endogenous ATP and its receptors. Furthermore, exogenous AT...Continue Reading
References
Jan 1, 1988·The Journal of Experimental Medicine·A F LopezM A Vadas
Sep 1, 1994·The Journal of Allergy and Clinical Immunology·B S Bochner, R P Schleimer
Feb 10, 1994·Journal of Immunological Methods·M IdeG J Gleich
Aug 1, 1996·The Journal of Allergy and Clinical Immunology·S HorieH Kita
Jul 3, 1998·American Journal of Respiratory Cell and Molecular Biology·M NagataW W Busse
Dec 20, 2000·FEBS Letters·D FerrariW Luttmann
Jul 27, 2001·Journal of Cellular Physiology·M IdzkoJ Norgauer
Apr 16, 2002·Science·Polly Matzinger
Apr 25, 2003·British Journal of Pharmacology·Marco IdzkoDavide Ferrari
Dec 22, 2004·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Keiko SaitoYoshio Sakamoto
Feb 4, 2006·American Journal of Respiratory Cell and Molecular Biology·Izumi KikuchiMakoto Nagata
Dec 16, 2006·Science·Yu ChenWolfgang G Junger
May 8, 2007·The Journal of Allergy and Clinical Immunology·Elizabeth A JacobsenJames J Lee
Jul 17, 2007·Nature Medicine·Marco IdzkoBart N Lambrecht
Nov 28, 2007·Annual Review of Pathology·Kenneth L Rock, Hajime Kono
Apr 4, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Simon P HoganMarc E Rothenberg
Nov 20, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M A M Willart, B N Lambrecht
Feb 28, 2009·Nature Reviews. Immunology·Philippa MarrackMichael W Munks
Oct 1, 2010·Allergy·T MüllerM Idzko
Nov 23, 2010·Nature Reviews. Immunology·Grace Y Chen, Gabriel Nuñez
Apr 9, 2011·Immunity·Mirjam KoolBart N Lambrecht
Oct 19, 2011·Respiratory Research·Yotaro TakakuMakoto Nagata
May 3, 2014·Nature Medicine·Christine SchauerMartin Herrmann
Citations
Aug 30, 2016·Chest·Amir PellegPeter J Barnes
Jan 9, 2018·Oncoimmunology·Gilda VarricchiGiovanna Schiavoni
Oct 12, 2018·Cancer Immunology, Immunotherapy : CII·Sonja C S SimonViktor Umansky
Sep 4, 2016·Oncotarget·Jue-Ping ShiHua Ren
Aug 1, 2020·Frontiers in Immunology·Davide FerrariMarco Idzko
Oct 16, 2019·Frontiers in Immunology·Julian M StarkJonathan M Coquet
Apr 14, 2020·Cancer Cell International·Li-Li FengXin Wang
Apr 25, 2020·Biomolecules·Kazuyuki Nakagome, Makoto Nagata
Oct 17, 2018·Frontiers in Immunology·Kazuyuki Nakagome, Makoto Nagata
Jun 20, 2017·Journal of Immunology Research·Fang ZhangYong Song
Oct 31, 2020·International Journal of Molecular Sciences·Renu BalyanNicholas R J Gascoigne