ATP-Evoked Intracellular Ca²⁺ Signaling of Different Supporting Cells in the Hearing Mouse Hemicochlea

Neurochemical Research
T HorváthT Zelles

Abstract

Hearing and its protection is regulated by ATP-evoked Ca(2+) signaling in the supporting cells of the organ of Corti, however, the unique anatomy of the cochlea hampers observing these mechanisms. For the first time, we have performed functional ratiometric Ca(2+) imaging (fura-2) in three different supporting cell types in the hemicochlea preparation of hearing mice to measure purinergic receptor-mediated Ca(2+) signaling in pillar, Deiters' and Hensen's cells. Their resting [Ca(2+)]i was determined and compared in the same type of preparation. ATP evoked reversible, repeatable and dose-dependent Ca(2+) transients in all three cell types, showing desensitization. Inhibiting the Ca(2+) signaling of the ionotropic P2X (omission of extracellular Ca(2+)) and metabotropic P2Y purinergic receptors (depletion of intracellular Ca(2+) stores) revealed the involvement of both receptor types. Detection of P2X2,3,4,6,7 and P2Y1,2,6,12,14 receptor mRNAs by RT-PCR supported this finding and antagonism by PPADS suggested different functional purinergic receptor population in pillar versus Deiters' and Hensen's cells. The sum of the extra- and intracellular Ca(2+)-dependent components of the response was about equal with the control ATP respo...Continue Reading

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Citations

Jun 14, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Rui YeJianxiong Li
Dec 14, 2016·PloS One·Dylan K Chan, Stephanie L Rouse
Mar 27, 2018·International Journal of Toxicology·David V GauvinRachel Tapp
Nov 22, 2016·Journal of Cellular Physiology·Rahul MittalXue Zhong Liu
Jun 21, 2019·International Journal of Molecular Sciences·László KölesTibor Zelles

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