PMID: 2499355Jun 15, 1989Paper

ATP-induced calcium mobilization in human neutrophils

Biochimica Et Biophysica Acta
M KurokiS Minakami

Abstract

Extracellular ATP at 10 microM increased the concentration of cytoplasmic free Ca2+ ( [Ca2+]i) 3-fold in human neutrophils. The [Ca2+]i was measured by fura-2 fluorescence. The effect was rapid but transient: [Ca2+]i reached a maximum within 10 s and then returned to its resting value after 2-3 min. The rise in [Ca2+]i elicited by ATP was unaffected by the removal of extracellular Ca2+, indicating that the primary source of Ca2+ is from intracellular stores. In contrast to ATP, neither ADP nor AMP, at concentrations as high as 100 microM, caused any detectable changes in [Ca2+]i. Among other nucleotide triphosphates tested, UTP was as effective as ATP in causing a transient rise in [Ca2+]i, and prevented a subsequent response to ATP. Similarly, ATP prevented a subsequent response to UTP but the second response could be obtained when the initially added ATP was removed by the addition of hexokinase. Phorbol myristate acetate, the activator of Ca2+, phospholipid-dependent protein kinase, completely inhibited the ATP-induced increases in [Ca2+]i without affecting the basal [Ca2+]i level. The results suggest that extracellular ATP stimulates human neutrophils by causing the release of calcium from intracellular storage pools by mec...Continue Reading

References

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Citations

May 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C SchöflJ A Gallagher
Aug 1, 1992·The International Journal of Biochemistry·T KitazonoS Minakami
Mar 1, 1997·General Pharmacology·B B Fredholm
Jan 1, 1990·Annals of the New York Academy of Sciences·E Rapaport
Jan 9, 2001·The Veterinary Journal·B L CoomberP A Gentry
Oct 30, 2014·Purinergic Signalling·Geoffrey Burnstock, Jean-Marie Boeynaems
Jun 13, 2002·Neuroreport·Jian-Zhong HanYi-Zhang Chen
Feb 22, 2002·Experimental Biology and Medicine·Joseph P GrandeKarl A Nath
Feb 19, 1992·Biochimica Et Biophysica Acta·C el-MoatassimJ C Mani
Oct 1, 1993·The Journal of Investigative Dermatology·J E SavageR Anderson
Oct 31, 1989·Biochemical and Biophysical Research Communications·F A GonzalezL A Heppel

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