ATP-Induced IL-1β Specific Secretion: True Under Stringent Conditions

Frontiers in Immunology
Monique StoffelsAnna Simon

Abstract

Interleukin-1β is a potent proinflammatory cytokine, of which processing and secretion are tightly regulated. After exposure to various stimuli, mononuclear phagocytes synthesize the inactive precursor (pro-IL-1β), which is then cleaved intracellularly by caspase-1 and secreted. A widely used method for in vitro secretion of IL-1β employs LPS-primed human peripheral blood monocytes. Subsequently, adenosine triphosphate (ATP) is added to the cells in order to trigger the P2X7 receptor resulting in processing and secretion of mature IL-1β. However, it is often reported that secretion is due to cytotoxic effects of ATP with P2X7 receptor-activation-related cell death. We have challenged this concept and demonstrate IL-1β specific secretion, since there is no increase in cell death and IL-1α and IL-18 are not released in the same cultures. More importantly we show that these conclusions can only be drawn under stringent experimental conditions.

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Citations

Feb 13, 2016·Cell Death and Differentiation·F Martín-SánchezP Pelegrín
Mar 16, 2017·Journal of Cellular Biochemistry·Mai OtsukaTatsuji Nishihara
Mar 9, 2017·Journal of Cellular and Molecular Medicine·Elisabeth NaschbergerNathalie Britzen-Laurent
Apr 4, 2019·The Journal of Biological Chemistry·Victor S TapiaDavid Brough
Sep 11, 2019·International Journal of Molecular Sciences·Fusheng JiangGuiyuan Lv
Apr 22, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yang YangScott R Johnstone
May 31, 2020·International Journal of Molecular Sciences·Rachel M KoldejDavid S Ritchie
Jul 13, 2016·The Journal of Biological Chemistry·Robert SteinSigrun Ruth Hofmann
Jul 24, 2018·The Tohoku Journal of Experimental Medicine·Van Minh HoNobuaki Shime
Jan 29, 2019·Frontiers in Immunology·Zayda L Piedra-QuinteroLeopoldo Santos-Argumedo
Aug 15, 2019·Journal of Translational Medicine·Alexandra Burr, Biju Parekkadan
Jun 12, 2020·Frontiers in Physiology·Amanda Ferreira NevesCarlos Amílcar Parada
Feb 25, 2017·Frontiers in Immunology·Giulio CavalliLorenzo Dagna
May 20, 2021·Scientific Reports·Shannon JamiesonAlison Tyson-Capper

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Methods Mentioned

BETA
ELISA
ELISAs
flow cytometry

Software Mentioned

CXP
GraphPad Prism
GraphPad

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