ATP-induced lysis of rat parotid secretory granules: possible role of ATP in exocytotic release

Journal of Supramolecular Structure
S G Oberg, M R Robinovitch

Abstract

Secretory vesicles isolated from a variety of mammalian tissues are known to lyse and thereby release their secretory products when exposed to ATP. This process, which will be termed ATP-induced lysis, has been studied most extensively using adrenal chromaffin-granule preparations. We report here that ATP causes the lysis of a highly purified preparation of rat parotid secretory granules. The rate of granule lysis was measured spectrophotometrically, and ATP-induced lysis was expressed as the increase in the rate of lysis (r = % lysis per min) when ATP was added. This lytic process was characterized with respect to pH, temperature, osmolarity, and the ionic composition of the media. ATP-induced lysis of parotid granules was found to have the following properties in common with the extensively characterized chromaffin-granule process: 1. It is a saturable function of ATP with half-maximal rates observed at 0.5 +/- 0.1 mM ATP. 2. It is temperature dependent, eg, r = 6.1 +/- 2.1%/min at 30 degrees C vs 12.2 +/- 2.5%/min at 37 degrees C. 3. It is inhibited in hyperosmotic media, eg, r = 5.3 +/- 0.3%/min at 0.3 OsM vs 0.8 +/- 0.2%/min at 0.4 OsM. 4. It shows a nucleotide preference of ATP = GTP greater than ADP greater than AMP grea...Continue Reading

References

Dec 1, 1976·The Journal of Membrane Biology·C Miller, E Racker
Mar 13, 1975·Biochimica Et Biophysica Acta·M R RobinovitchD L Kauffman
Jun 11, 1968·Biochimica Et Biophysica Acta·T MatsudaH Yoshida
Oct 1, 1970·The Journal of Cell Biology·B Hellman, I B Täljedal
Apr 1, 1973·Analytical Biochemistry·N KirshnerM Schramm
Apr 1, 1971·Japanese Journal of Pharmacology·H IshidaH Yoshida
Dec 1, 1963·Biochemical Pharmacology·R J WURTMAN, J AXELROD

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Citations

Jan 1, 1983·Journal of the Autonomic Nervous System·D Njus
Jan 1, 1986·Biochimica Et Biophysica Acta·D NjusG J Harnadek
Feb 1, 1987·Journal of Dental Research·K T IzutsuM Goddard

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