ATP induces Ca2+ release from IP3-sensitive Ca2+ stores exclusively in large DRG neurones

Neuroreport
N SvicharP Kostyuk

Abstract

PURINORECEPTOR-MEDIATED intracellular Ca2+ release was studied in freshly isolated adult mouse dorsal root ganglia (DRG) neurones. The cytoplasmic Ca2+ concentration ([Ca2+]i) was measured using indo-1-based microfluorimetry. The application of 100 microM ATP in Ca(2+)-free solution triggered an increase in [Ca2+]i in 93% of large DRG neurones but in no small ones. The ATP-induced [Ca2+]i transients in large neurones were inhibited by cells incubation with thapsigargin or by intracellular dialysis with heparin-containing solution. The ATP-triggered increase in [Ca2+]i was not mimicked by adenosine and was blocked by suramin, suggesting the involvement of metabotropic (PZY) purinoreceptors. We conclude that large (proprioceptive) DRG neurones express PZY purinoreceptors linked to the inositol 1,4,5-triphosphate-Ca2+ intracellular signal transduction cascade, whereas small (nociceptive) DRG neurones are devoid of such a mechanism.

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Citations

Apr 12, 2008·Purinergic Signalling·Zoltan Gerevich, Peter Illes
Apr 12, 2008·Purinergic Signalling·Kazuhide InoueSchuichi Koizumi
Apr 12, 2008·Purinergic Signalling·Kazuhide Inoue
Aug 2, 2002·European Journal of Pharmacology·Alexej Verkhratsky, Ole H Petersen
Jan 25, 2003·Cell Calcium·A Verkhratsky
Nov 7, 1998·Brain Research. Developmental Brain Research·U Lalo, P Kostyuk
Jan 4, 2001·Trends in Neurosciences·R D Fields, B Stevens
Feb 13, 1999·Progress in Neurobiology·M J Millan
Jun 24, 2009·BMC Neuroscience·Manoj Kumar JaiswalBernhard U Keller
Sep 30, 2003·The Journal of Physiology·C KennedyE G Rowan
May 11, 2010·Brain Research Reviews·Claudia Verderio, Michela Matteoli
Nov 23, 2007·Cell Calcium·Stefania CerutiMaria P Abbracchio
Oct 18, 2005·Pharmacology & Therapeutics·Geoffrey Burnstock
May 11, 2015·Purinergic Signalling·Marek CieślakMichał Komoszyński
Apr 15, 2004·Pain·Cheryl L StuckyDerek C Molliver
Feb 17, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·H MassaeliG N Pierce
Feb 3, 2004·Journal of Cellular and Molecular Medicine·A Verkhratsky, E C Toescu
Mar 30, 2010·Acta Physiologica·G BurnstockA Verkhratsky
Aug 12, 2000·Journal of Neurophysiology·M RenganathanS G Waxman
May 3, 2006·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Kazuhide Inoue
Mar 29, 2001·Physiological Reviews·O P Hamill, B Martinac
Mar 2, 2006·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Kazuhide Inoue, Makoto Tsuda

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