ATP is released from rabbit urinary bladder epithelial cells by hydrostatic pressure changes--a possible sensory mechanism?

The Journal of Physiology
D R FergusonT J Burton

Abstract

1. The responses of rabbit urinary bladder to hydrostatic pressure changes and to electrical stimulation have been investigated using both the Ussing chamber and a superfusion apparatus. These experiments enabled us to monitor changes in both ionic transport across the tissue and cellular ATP release from it. 2. The urinary bladder of the rabbit maintains an electrical potential difference across its wall as a result largely of active sodium transport from the urinary (mucosal) to the serosal surface. 3. Small hydrostatic pressure differences produced by removal of bathing fluid from one side of the tissue caused reproducible changes in both potential difference and short-circuit current. The magnitude of these changes increases as the volume of fluid removed increases. 3. Amiloride on the mucosal (urinary), but not the serosal, surface of the membrane reduces the transepithelial potential difference and short-circuit current with an IC50 of 300 nM. Amiloride reduces the size of, but does not abolish, transepithelial potential changes caused by alterations in hydrostatic pressure. 4. Field electrical stimulation of strips of bladder tissue produces a reproducible release of ATP. Such release was demonstrated to occur largely fr...Continue Reading

References

Aug 27, 1976·The Journal of Membrane Biology·S A Lewis, J M Diamond
Sep 1, 1988·Quarterly Journal of Experimental Physiology : an International Journal of the Physiological Society·A J NimmoE M Whitaker
Oct 1, 1988·The Journal of Membrane Biology·T R Kleyman, E J Cragoe
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R E Fyffe, E R Perl
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·F Nakamura, S M Strittmatter
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y WangJ G Fitz
Mar 1, 1997·The American Journal of Physiology·R Grygorczyk, J W Hanrahan

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Citations

Apr 23, 2002·The Prostate·Jun Hee KimSung Joon Kim
Mar 23, 2011·Der Urologe. Ausg. A·T BschleipferW Weidner
Apr 26, 2013·Der Urologe. Ausg. A·T BschleipferW Weidner
Nov 24, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Naoki YoshimuraPradeep Tyagi
Mar 4, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Atsushi OtsukaSeiichiro Ozono
Mar 2, 2010·Archives of Gynecology and Obstetrics·Kiran Ashok, Alex Wang
May 2, 2006·Pflügers Archiv : European journal of physiology·Joel R GeverAnthony P D W Ford
Apr 29, 2006·Pflügers Archiv : European journal of physiology·Heike FrankePeter Illes
Jun 4, 2011·Pflügers Archiv : European journal of physiology·Sarah KesslerMartin Fronius
May 20, 2005·Cell and Tissue Research·Raymond E PapkaMegan Storey-Workley
Mar 7, 2008·Journal of Neural Transmission·A Apostolidis, C J Fowler
Oct 24, 2007·Molecular Neurobiology·Kerstin WirknerPeter Illes
Mar 15, 2005·European Journal of Pharmacology·Timothy J BurtonDouglas R Ferguson
Mar 7, 2013·European Journal of Pharmacology·Makiko IshimaruShuji Kojima
Nov 5, 2003·Gastroenterology·Gregory WynnGeoffrey Burnstock
Sep 21, 2000·European Journal of Pharmacology·T J BurtonD R Ferguson
Jul 30, 2002·European Journal of Pharmacology·Timothy J BurtonDouglas R Ferguson
May 15, 2002·Urology·Karl-Erik Andersson
Dec 21, 2002·Urology·Karl-Erik Andersson, Petter Hedlund
Jun 28, 2000·Journal of the Autonomic Nervous System·S G Hamilton, S B McMahon
Apr 3, 2001·Trends in Pharmacological Sciences·G Burnstock
Jun 19, 2001·Progress in Neurobiology·P M DunnG Burnstock
Nov 1, 2001·Journal of Pharmacological and Toxicological Methods·P A Longhurst, B Uvelius
Feb 14, 2006·Nature Clinical Practice. Urology·Toby C Chai, Susan Keay
Jan 11, 2007·Nature Clinical Practice. Urology·Lori A Birder, William C de Groat
May 21, 2008·Nature Reviews. Neuroscience·Clare J FowlerWilliam C de Groat
Mar 5, 2003·BJU International·O J WisemanD N Landon
Jun 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ying-Jie PengNanduri R Prabhakar
Jan 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jayasri NanduriNanduri R Prabhakar
Apr 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Guoxiang YuanNanduri R Prabhakar

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