PMID: 2126013Dec 1, 1990Paper

ATP is required for receptor-mediated endocytosis in intact cells

The Journal of Cell Biology
S L Schmid, L L Carter

Abstract

We have demonstrated a requirement for cellular ATP in the receptor-mediated endocytosis of transferrin. This has been accomplished using a novel assay for endocytosis based on acquisition of resistance to the membrane impermeable reducing agent, glutathione (GSH). Diferric-transferrin was conjugated to biotin via a cleavable disulfide bond and iodinated. Internalization of 125I-biotin-S-S-transferrin (125I-BSST) was quantitated by adsorption to avidin-Sepharose after treatment of cells with GSH. Receptor-mediated endocytosis of 125I-BSST was severely inhibited in ATP-depleted cells. Similar results were obtained when ATP was depleted by incubation of cells either under a N2-atmosphere or in the presence of NaN3 and NaF. The latter treatment, alone, also resulted in a loss of surface transferrin receptors which could not be correlated to reductions in cellular ATP. In contrast to the acquisition of GSH resistance, the apparent internalization of 125I-BSST as assessed by inaccessibility to antitransferrin antibodies reached control levels in ATP-depleted cells. Our biochemical and morphological data suggested that, although ATP is required for receptor-mediated endocytosis, in ATP-depleted cells ligands can become efficiently se...Continue Reading

References

Jan 1, 1977·Annual Review of Biochemistry·S C SilversteinZ A Cohn
Jan 1, 1989·Annual Review of Cell Biology·J Gruenberg, K E Howell
Mar 1, 1989·The Journal of Cell Biology·E SmytheG Warren
May 1, 1989·The Journal of Cell Biology·D T MahaffeyR G Anderson
Jan 1, 1989·Journal of Cell Science. Supplement·M S Moore, R G Anderson
Aug 1, 1989·Current Opinion in Cell Biology·S A MorrisE Ungewickell
Jan 1, 1985·Annual Review of Cell Biology·J L GoldsteinW J Schneider
May 1, 1987·Science·M S MooreR G Anderson
Jan 1, 1987·Annual Review of Biophysics and Biophysical Chemistry·B M Pearse, R A Crowther
Aug 1, 1987·The Journal of Cell Biology·K SandvigB van Deurs
Apr 5, 1988·European Journal of Biochemistry·P G Woodman, G Warren
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W A Braell
Aug 7, 1983·Journal of Theoretical Biology·D N McKinley
Nov 1, 1983·The Journal of Cell Biology·S Zaremba, J H Keen
Aug 1, 1984·The Journal of Cell Biology·D M SchlossmanJ E Rothman
Sep 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M C WillinghamI H Pastan
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A C KingP Cuatrecasas

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Citations

Sep 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S L Schmid
Dec 1, 1994·Cell and Tissue Research·M Kressel, P Groscurth
Jan 1, 1992·Molecular and Biochemical Parasitology·K Haldar, L Uyetake
Aug 1, 1991·Current Opinion in Cell Biology·I S Trowbridge
Oct 16, 2008·Biochemistry·Cindy A Puckett, Jacqueline K Barton
Jun 26, 2009·Journal of the American Chemical Society·Jingyi ChenIwao Ojima
Aug 14, 2009·Journal of the American Chemical Society·Laila DafikKrishna Kumar
Jan 25, 2013·Molecular Therapy. Nucleic Acids·Samantha E WilnerMatthew Levy
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J F Hare, K Taylor
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y KatoY Sugiyama
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·S M VoglmaierS H Snyder
Sep 1, 1991·The Journal of Cell Biology·S L Schmid, E Smythe
Sep 1, 1991·The Journal of Cell Biology·H C LinR G Anderson
Dec 1, 1991·The Journal of Cell Biology·F J Stafford, J S Bonifacino
Dec 1, 1992·The Journal of Cell Biology·E SmytheS L Schmid
Aug 1, 1993·The Journal of Cell Biology·A M van der BliekS L Schmid
Feb 1, 1994·The Journal of Cell Biology·E SmytheS E Moss
Nov 1, 1994·The Journal of Cell Biology·H DamkeS L Schmid
Jun 25, 2004·Molecular Biology of the Cell·Edmund NestiAnthony D Morielli
Dec 17, 2004·Molecular Biology of the Cell·Defne YararSandra L Schmid
Jun 12, 2009·Molecular Biology of the Cell·Joshua S ChappieSandra L Schmid
Dec 18, 1991·European Journal of Biochemistry·E Smythe, G Warren
Nov 30, 2007·Journal of Virology·Jillian M RojekStefan Kunz
Jan 1, 1997·Annual Review of Biochemistry·S L Schmid
May 24, 2012·International Journal of Nanomedicine·Pramod K Avti, Balaji Sitharaman
Feb 11, 2014·Biomacromolecules·Federica SgolastraGregory N Tew
Jan 30, 2014·Molecular Therapy. Nucleic Acids·David PorcianiFabio Beltram
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·P M DayJ R Bennink
Jan 1, 1994·Parasitology Research·A L AffonsoW De Souza
Oct 12, 2010·Molecular Membrane Biology·Devika B Chithrani
Mar 27, 2001·The Journal of Cell Biology·E HillE Smythe
Feb 7, 2013·Colloids and Surfaces. B, Biointerfaces·Kaushik Mallick, André M Strydom
Nov 22, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mitsuo YamadaShun'ichi Kuroda

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