PMID: 3771635Oct 1, 1986Paper

ATP keeps exocytosis sites in a primed state but is not required for membrane fusion: an analysis with Paramecium cells in vivo and in vitro

The Journal of Cell Biology
J Vilmart-SeuwenH Plattner

Abstract

We have tried to specify a widespread hypothesis on the requirement of ATP for exocytosis (membrane fusion). With Paramecium tetraurelia cells, synchronously (approximately 1 s) exocytosing trichocysts, ATP pools have been measured in different strains, including wild type cells, "non-discharge" (nd), "trichless" (tl), and other mutations. The occurrence of a considerable and rapid ATP consumption also in nd and tl mutations as well as its time course (with a maximum 3-5 s after exocytosis) in exocytosis-competent strains does not match the actual extent of exocytosis performance. However, from in vivo as well as from in vitro experiments, we came to the conclusion that ATP might be required to keep the system in a primed state and its removal might facilitate membrane fusion. (For the study of exocytosis in vitro we have developed a new system, consisting of isolated cortices). In vivo as well as in vitro exocytosis is inhibited by increased levels of ATP or by a nonhydrolyzable ATP analogue. In vitro exocytosis is facilitated in ATP-free media. In vivo-microinjected ATP retards exocytosis in response to chemical triggers, whereas microinjected apyrase triggers exocytosis without exogenous trigger. Experiments with this system...Continue Reading

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Citations

Jul 12, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Helmut Plattner
Sep 1, 1991·The Journal of Membrane Biology·D J Lew, S M Simon
Mar 22, 2001·Advanced Drug Delivery Reviews·U BickelW M Pardridge
Jun 24, 2000·Biochimie·L VayssiéJ Cohen
May 1, 1991·The Journal of Cell Biology·T WhalleyM Whitaker
Jun 10, 1996·Biochimica Et Biophysica Acta·M Linial, D Parnas
Mar 16, 2006·Traffic·Christina SchildeRoland Kissmehl
Sep 5, 2013·The Journal of Eukaryotic Microbiology·Helmut Plattner
Jul 1, 1989·The Journal of protozoology·B H Satir
Sep 1, 1987·Journal of Neurochemistry·G Ahnert-Hilger, M Gratzl
Aug 14, 2003·Biochimica Et Biophysica Acta·Helmut Plattner, Roland Kissmehl
Aug 14, 2003·Biochimica Et Biophysica Acta·Joseph A Szule, Jens R Coorssen
Oct 18, 2016·Frontiers in Cell and Developmental Biology·Jorge Bernardino de la SernaMarek Cebecauer
Oct 22, 2015·Biological Reviews of the Cambridge Philosophical Society·Helmut Plattner
Feb 1, 1989·Bioscience Reports·P E Tatham, B D Gomperts
Jan 27, 1998·The Journal of Biological Chemistry·V V ChestkovS S Vogel
Oct 22, 2003·Journal of Biomolecular Screening·Janet WilsonAlexander Scheer
Sep 24, 2002·Journal of Cell Science·Roland KissmehlJean Cohen
Jul 22, 1991·Biochimica Et Biophysica Acta·R D Burgoyne

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