ATP receptor-induced Ca2+ transients in cardiac myocytes: sources of mobilized Ca2+.

The American Journal of Physiology
M B De Young, A Scarpa

Abstract

Addition of micromolar concentrations of extracellular ATP to adult rat cardiac ventricular myocytes increases cytosolic Ca2+ concentration ([Ca2+]). Experiments were performed on fura-2-loaded myocytes to determine whether the [Ca2+] rise was due to Ca2+ influx, release of Ca2+ from the sarcoplasmic reticulum (SR), or a combination of both. BAY K 8644 and nifedipine affected ATP-induced [Ca2+] transients, indicating involvement of voltage-sensitive Ca2+ channels. Addition of ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) or Ca2+ channel blockers significantly reduced cytosolic [Ca2+] changes due to addition of ATP or KCl without depleting Ca2+ stores (shown by ionomycin treatment in a Ca2+-free medium), demonstrating that these responses require Ca2+ influx. Depletion of intracellular Ca2+ stores by caffeine or ryanodine also diminished cytosolic [Ca2+] responses, indicating that a portion of the increased cytosolic [Ca2+] is due to Ca2+ release from SR. Norepinephrine potentiates the ATP-Ca2+ response, and this effect was not inhibited by depletion of intracellular stores. Although the data show that there are two Ca2+ sources in the cytosolic Ca2+ response to ATP, the pattern is also consistent ...Continue Reading

References

Jul 1, 1986·Journal of Molecular and Cellular Cardiology·R W TsienM C Nowycky
Jan 15, 1986·The Biochemical Journal·J L Gordon
Mar 13, 1987·Biochemical and Biophysical Research Communications·S PhaneufJ C Cavadore
Sep 1, 1987·The Journal of Physiology·R G Hansford, E G Lakatta
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G CallewaertM Morad
Nov 30, 1987·Biochemical and Biophysical Research Communications·M H DavisG P Brierley
Feb 1, 1986·Pflügers Archiv : European journal of physiology·H LotanY Lass
Aug 25, 1983·Nature·C E Jahr, T M Jessell

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Citations

Mar 1, 1993·Journal of Cellular Physiology·S RahaJ K Reed
Feb 1, 1993·Circulation Research·D S Damron, M Bond
Aug 1, 1993·British Journal of Pharmacology·L MantelliF Ledda
Feb 23, 2000·Journal of Cardiovascular Pharmacology and Therapeutics·M S NijjarN S Dhalla
Mar 29, 2001·Physiological Reviews·G Vassort
Apr 5, 2001·American Journal of Physiology. Cell Physiology·E M HurK T Kim
Jan 18, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Harjot K Saini, Naranjan S Dhalla
Sep 13, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Harjot K SainiNaranjan S Dhalla
Dec 21, 2014·Purinergic Signalling·Geoffrey Burnstock, Amir Pelleg
Nov 30, 1992·Annals of the New York Academy of Sciences·B GiannattasioA Scarpa
Apr 1, 1994·British Journal of Pharmacology·M KanedaK Doi
Jun 19, 2001·American Journal of Physiology. Heart and Circulatory Physiology·Q Mei, B T Liang
May 8, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Alexander YangBruce T Liang
Nov 1, 1990·Pflügers Archiv : European journal of physiology·F Scamps, G Vassort
Jul 1, 1991·Circulation·V Ralevic, G Burnstock
Dec 31, 1997·The American Journal of Physiology·E PodraskyB T Liang
Jun 1, 1993·Pflügers Archiv : European journal of physiology·R NitschkeR Greger
Feb 23, 2000·Journal of Cardiovascular Pharmacology and Therapeutics·S MusatN S Dhalla
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Alex V BirkHazel H Szeto
Oct 19, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B HuB T Liang
Apr 12, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Harjot K SainiNaranjan S Dhalla
Nov 29, 2013·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Juliano Quintella Dantas RodriguesAron Jurkiewicz
Apr 1, 1991·American Journal of Respiratory Cell and Molecular Biology·G R Dubyak
Apr 6, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·D M ZhaoH Nakamura
Apr 5, 2001·American Journal of Physiology. Cell Physiology·Q Y Liu, R L Rosenberg
Dec 30, 1994·Biochimica Et Biophysica Acta·C A HansenJ D Robishaw
Aug 1, 1991·Molecular and Cellular Endocrinology·K Törnquist
Feb 19, 1992·Biochimica Et Biophysica Acta·C el-MoatassimJ C Mani
Sep 1, 1994·Trends in Cardiovascular Medicine·G VassortF Scamps

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