ATP regulation of a large conductance voltage-gated cation channel in rough endoplasmic reticulum of rat hepatocytes

Archives of Biochemistry and Biophysics
Manoochehr AshrafpourR Saghiri

Abstract

ATP-sensitive K+ channels play an important role in regulating membrane potential during metabolic stress. In this work we report the effect of ATP and ADP-Mg on a K+ channel present in the membrane of rough endoplasmic reticulum (RER) from rat hepatocytes incorporated into lipid bilayers. Channel activity was found to decrease in presence of ATP 100 microM on the cytoplasmic side and was totally inhibited at ATP concentrations greater than 0.25mM. The effect appeared voltage dependent, suggesting that the ATP binding site was becoming available upon channel opening. Channel activity was suppressed by the nonhydrolyzable ATP analog (ATPgammaS), ruling out a phosphorylation-based mechanism. Notably addition of 2.5mM ADP-Mg to the cytosolic side increased the channel open probability at negative potentials. We conclude that the large conductance voltage-gated cation channel in RER of rat hepatocytes is an ATP and ADP sensitive channel likely to be involved in cellular processes such as Ca(2+) signaling or control of membrane potential across the endoplasmic reticulum membrane.

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Citations

Jun 12, 2012·Pflügers Archiv : European journal of physiology·M AshrafpourH Sharifi
Aug 30, 2012·The Journal of Physiology·Harpreet SinghLigia Toro
Mar 13, 2016·Biochimica Et Biophysica Acta·Vanessa ChecchettoIldiko Szabo
Nov 16, 2018·British Journal of Pharmacology·Luigi LeanzaIldiko Szabo

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