ATP release by way of connexin 36 hemichannels mediates ischemic tolerance in vitro

Biochemical and Biophysical Research Communications
Sarah C SchockCharlie S Thompson

Abstract

Spreading depression (SD) is a self-propagating wave of neuronal and glial depolarization that may occur in virtually any gray matter region in the brain. One consequence of SD is an increased tolerance to ischemia. It has been shown that during cortical SD ATP is released into the extracellular space and activation of purinergic receptors leads to the induction of ischemic tolerance. In the present study we show that depolarization of cultured neurons induces ischemic tolerance which is mediated by purinergic receptor activation. Depolarization causes the release of ATP into the extracellular medium, which may be prevented by treatment with the connexin hemichannel blockers flufenamic acid and quinine, but not the pannexin hemichannel blocker carbenoxolone. Knockdown of connexin 36 expression by siRNA greatly reduces the amount of ATP released during depolarization and the subsequent degree of ischemic tolerance. We conclude that during depolarization neurons release ATP by way of connexin 36 hemichannels.

References

Oct 1, 1994·Trends in Neurosciences·H Zimmermann
Sep 1, 1995·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J HamadaM Reivich
Jan 1, 1994·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·W D DietrichM Rosenthal
Mar 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·K MatsushimaA M Hakim
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·M L CotrinaM Nedergaard
Jan 4, 2001·Trends in Neurosciences·R D Fields, B Stevens
Jan 16, 2002·The Journal of Biological Chemistry·Charles E StoutAndrew C Charles
Jun 18, 2002·Cell Communication & Adhesion·C C NausT Nakase
Jun 18, 2002·Journal of Neurochemistry·Beáta SperlághE Sylvester Vizi
Aug 12, 2004·FEBS Letters·Li BaoGerhard Dahl
Dec 13, 2005·The EMBO Journal·Elke De VuystLuc Leybaert
Dec 21, 2005·Neuron·Chris G DullaSusan A Masino
May 2, 2006·Advances in Cardiology·Rainer Schulz, Gerd Heusch

❮ Previous
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Citations

Oct 15, 2008·Pflügers Archiv : European journal of physiology·Eliana ScemesPaolo Meda
Apr 25, 2012·Purinergic Signalling·Eduardo R Lazarowski
Aug 7, 2012·Acta Pharmacologica Sinica·Nicholas L WeilingerRoger J Thompson
Feb 7, 2009·Cell Death and Differentiation·E DecrockL Leybaert
Sep 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Cristina RosetiFabrizio Eusebi
Jan 15, 2010·The Journal of Biological Chemistry·Andrew P FeranchakJ Gregory Fitz
Dec 17, 2008·The Journal of Biological Chemistry·Vytas K VerselisMiduturu Srinivas
Aug 13, 2008·The Journal of General Physiology·Vytas K Verselis, Miduturu Srinivas
Nov 26, 2008·The Journal of General Physiology·Roman A RomanovStanislav S Kolesnikov
Mar 15, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·David LuPaul A Insel
Oct 1, 2010·American Journal of Physiology. Cell Physiology·Kurt A SchalperJuan C Sáez
Feb 6, 2009·American Journal of Physiology. Cell Physiology·Domenick A ProsdocimoGeorge R Dubyak
Mar 20, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Masahito KawamuraSusan A Masino
Dec 15, 2012·Trends in Neurosciences·Andrei B Belousov, Joseph D Fontes
Sep 13, 2011·Progress in Neurobiology·Geoffrey BurnstockPeter Illes
Mar 17, 2010·Journal of Neuroscience Methods·Abdallah HayarEdgar Garcia-Rill
Dec 22, 2009·Trends in Neurosciences·Brian A MacVicar, Roger J Thompson
Oct 15, 2013·Glia·Anna Nualart-MartiCarles Solsona
Aug 1, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Catheleyne D'hondtBernard Himpens
Jul 17, 2012·Biochimica Et Biophysica Acta·Nan WangLuc Leybaert
Nov 16, 2013·BioMed Research International·José Luis VegaJuan Carlos Sáez
Aug 19, 2015·Frontiers in Cellular Neuroscience·Mauricio A RetamalCarlos González
Jun 19, 2015·Cellular and Molecular Life Sciences : CMLS·David J HodsonNathalie C Guérineau
Jun 30, 2015·Cellular and Molecular Life Sciences : CMLS·Elke DecrockLuc Leybaert
Apr 18, 2015·Frontiers in Cellular Neuroscience·Juan A OrellanaJuan C Sáez
Dec 6, 2014·Neuroscience·T D Montero, J A Orellana
Jul 26, 2012·Biochemical and Biophysical Research Communications·D UrbanT Noll
Aug 16, 2011·Biochimica Et Biophysica Acta·Antonio Rodríguez-SinovasDavid Garcia-Dorado
Jun 28, 2011·Biochimica Et Biophysica Acta·Eliana Scemes
Jul 26, 2016·Frontiers in Neuroscience·Erica F DiezmosLu Liu
Oct 8, 2016·Frontiers in Molecular Neuroscience·Rosario Gajardo-GómezJuan A Orellana
Feb 7, 2017·Nature Communications·Tongde WuRobert Tarran
Jun 27, 2017·Pharmacology & Therapeutics·Akiyuki NishimuraMotohiro Nishida
Jan 27, 2017·BMC Cell Biology·Andrei B BelousovChristian C Naus
Feb 20, 2014·FEBS Letters·Alexander W Lohman, Brant E Isakson
Feb 2, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Tetsuji MiuraToshiyuki Yano

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