ATP-sensitive K⁺ channels contribute to the protective effects of exogenous hydrogen sulfide against high glucose-induced injury in H9c2 cardiac cells

International Journal of Molecular Medicine
Weijie LiangXinxue Liao

Abstract

Hyperglycemia, as well as diabetes mellitus, has been shown to impair ATP-sensitive K+ (KATP) channels in human vascular smooth muscle cells. Hydrogen sulfide (H2S) is also known to be an opener of KATP channels. We previously demonstrated the cardioprotective effects exerted by H2S against high-glucose (HG, 35 mM glucose)-induced injury in H9c2 cardiac cells. As such, we hypothesized that KATP channels play a role in the cardioprotective effects of H2S against HG-induced injury. In this study, to examine this hypothesis, H9c2 cardiac cells were treated with HG for 24 h to establish a model of HG-induced insults. Our findings revealed that treatment of the cells with HG markedly decreased the expression level of KATP channels. However, the decreased expression of KATP channels was reversed by the treatment of the cells with 400 µM sodium hydrogen sulfide (NaHS, a donor of H2S) for 30 min prior to exposure to HG. Additionally, the HG-induced cardiomyocyte injuries, including cytotoxicity, apoptosis, oxidative stress and mitochondrial damage, were ameliorated by treatment with NaHS or 100 µM diazoxide (a mitochondrial KATP channel opener) or 50 µM pinacidil (a non-selective KATP channel opener) for 30 min prior to exposure to HG,...Continue Reading

References

Nov 1, 1986·Pflügers Archiv : European journal of physiology·G TrubeT Ohno-Shosaku
Apr 12, 1996·The Journal of Biological Chemistry·K D GarlidP A Schindler
May 9, 1998·Kidney International·M SmogorzewskiS G Massry
Jan 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Cevher OzcanAndre Terzic
Nov 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rui Wang
Feb 5, 2003·Progress in Biophysics and Molecular Biology·Susumu Seino, Takashi Miki
Jun 24, 2003·Diabetologia·F M Gribble, F Reimann
Aug 14, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Garrett J Gross, Jason N Peart
Sep 25, 2003·Physiological Reviews·Derek M Yellon, James M Downey
Dec 20, 2003·Biochemical and Biophysical Research Communications·Bin GengChaoshu Tang
Oct 16, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Hiroyuki KinoshitaYoshio Hatano
Oct 6, 2005·The Journal of Pharmacology and Experimental Therapeutics·Jin-Song BianPhilip Keith Moore
Apr 1, 2006·The Journal of Pharmacology and Experimental Therapeutics·Hiroyuki KinoshitaYoshio Hatano
Jul 13, 2006·Gastroenterology·Stefano FiorucciJohn L Wallace
Aug 4, 2006·The New England Journal of Medicine·Ewan R PearsonUNKNOWN Neonatal Diabetes International Collaborative Group
Jul 21, 2007·Journal of Applied Physiology·L V ZingmanA Terzic
Oct 20, 2007·Nature Reviews. Drug Discovery·Csaba Szabó
Sep 23, 2009·Clinical and Experimental Pharmacology & Physiology·Si-Lin ChenJian-Qiang Feng
Jul 29, 2010·Physiological Reviews·Thomas P FlaggColin G Nichols
Mar 29, 2011·Journal of Molecular and Cellular Cardiology·Leonid V ZingmanDenice M Hodgson-Zingman
Aug 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kunihiro SuzukiCsaba Szabo
Nov 15, 2011·Biochemical and Biophysical Research Communications·Zhiyong ZhuLeonid V Zingman
Dec 12, 2012·The Journal of Biological Chemistry·Ana SierraDenice M Hodgson-Zingman
Jan 9, 2013·Molecular and Cellular Biology·Yuan ZhangZhi-Sheng Jiang
Jan 23, 2013·International Journal of Molecular Medicine·Runmin GuoJianqiang Feng
Mar 13, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Bridgette F PeakeJohn W Calvert
Dec 21, 2013·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Runmin GuoJianqiang Feng

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Citations

Feb 22, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Tao DingHaiyan Hu
Jun 17, 2020·Journal of Cellular Physiology·Yuqi ChenXiang Zhou
Feb 6, 2020·Journal of Clinical Medicine·Elena GrossiniCarolina Monaco
Nov 13, 2018·Frontiers in Physiology·Maria Consiglia TrottaClara Di Filippo
Mar 3, 2020·Frontiers in Pharmacology·Yun ChenGuoliang Meng
Oct 9, 2020·Human & Experimental Toxicology·Seong-Ho OkJu-Tae Sohn
Mar 9, 2021·Frontiers in Cell and Developmental Biology·Shizhen ZhaoHonggang Wang
Jun 24, 2020·The International Journal of Biochemistry & Cell Biology·Daria RotkoBogusz Kulawiak

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