PMID: 6108987Jul 1, 1980Paper

ATP-stimulated Ca2+ transport into cholinergic Torpedo synaptic vesicles

Journal of Neurochemistry
D M MichaelsonI Angel

Abstract

Activation of the Ca2+/Mg2+ ATPase associated with highly purified Torpedo synaptic vesicles results in 45Ca2+ uptake. The accumulated 45Ca2+ is released by hypoosmotic buffer and by the Ca2+ ionophore A23187. Density-gradient centrifugation and permeation chromatography reveal that vesicular acetylcholine and the membrane-bound 45Ca2+ co-migrate, thus implying that 45Ca2+ is transported into cholinergic vesicles. ATP-dependent 45Ca2+ uptake follows saturation kinetics, with KmCa2+ = 50 microM, and Vmax = 3 +/- 0.3 nmol Ca2+/mg protein/min. Treatment of the vesicles with mersalyl, dicyclohexylcarbodiimide, and quercetin leads to inactivation of the Ca2+/Mg2+ ATPase and to comparable inhibition of 45Ca2+ transport. Ruthenium red and ouabain have no effect on either of these activities. Nigericin in the presence of external K+ is a potent inhibitor of 45Ca2+ translocation, whereas gramicidin activates transport. The proton translocator carbonylcyanide p-trifluoromethoxy-phenylhydrazone (FCCP) and FCCP + the ionophore valinomycin partially inhibit 45Ca2+ transport. By contrast, the above ionophores do not affect Ca2+/Mg2+ ATPase activity. Tentative mechanisms for ATP-dependent Ca2+ transport into cholinergic synaptic vesicles and ...Continue Reading

References

Nov 1, 1975·Analytical Biochemistry·G A KimmichJ S Brand
Apr 28, 1978·Annals of the New York Academy of Sciences·M P BlausteinN K Kendrick
Jan 1, 1979·Annual Review of Neuroscience·R B KellyJ A Wagner
Jan 1, 1975·Annual Review of Biophysics and Bioengineering·D H MacLennan, P C Holland
May 15, 1978·FEBS Letters·H Rahamimoff, E Abramovitz
Aug 15, 1978·FEBS Letters·H Rahamimoff, E Abramovitz
Jun 13, 1979·Biochemical and Biophysical Research Communications·J E Rothlein, S M Parsons
Jan 1, 1978·Journal of Neurochemistry·D M Michaelson, M Sokolovsky
Jan 20, 1977·Nature·N C KendrickR W Ratzlaff
Sep 1, 1978·Journal of Neurochemistry·A E Halaris, E M DeMet
Nov 8, 1976·Biochemical and Biophysical Research Communications·D M Michaelson, M Sokolovsky
Oct 1, 1976·Journal of Neurochemistry·J B Suszkiw
Jan 1, 1971·Annual Review of Microbiology·P J Henderson

❮ Previous
Next ❯

Citations

Dec 1, 1982·Journal of Bioenergetics and Biomembranes·R G Sorensen, H R Mahler
Feb 1, 1990·The Histochemical Journal·D FreihöferH Rahmann
Apr 29, 1998·Molecular Neurobiology·M Israël, Y Dunant
Jan 1, 1991·Life Sciences·H M HuangG Gibson
Oct 1, 1988·Trends in Neurosciences·M P Blaustein
Oct 1, 1988·Trends in Neurosciences·J MeldolesiT Pozzan
Jan 1, 1987·Neurochemistry International·H StelzlH Zimmermann
Jul 1, 1987·Neurobiology of Aging·G E Gibson, C Peterson
Jan 1, 1982·Progress in Neurobiology·P A KometianiE E Klein
Jan 1, 1986·Progress in Neurobiology·Y Dunant
Jan 1, 1993·Neuroscience·A Parducz, Y Dunant
Sep 1, 1993·Neuroscience·F González-Aguilar
Jan 1, 1993·Journal of Physiology, Paris·Y Dunant, M Israël
Jan 1, 1995·Journal of Physiology, Paris·Y Dunant, M Israël
Apr 24, 1999·Trends in Neurosciences·P FossierG Baux
Apr 27, 1999·Brain Research. Molecular Brain Research·P P GonçalvesM G Vale
Oct 16, 1999·Brain Research. Molecular Brain Research·P P GonçalvesM G Vale
Jul 9, 1998·Neuroscience Letters·P P GonçalvesM G Vale
Mar 17, 2000·Brain Research. Brain Research Protocols·P P GonçalvesM G Vale
Apr 13, 2000·The European Journal of Neuroscience·M JuhaszovaE F Stanley
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A Rephaeli, S M Parsons
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S B AndrewsT S Reese
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·N P Issa, A J Hudspeth
Jan 1, 1983·Annual Review of Biochemistry·L F Reichardt, R B Kelly
Sep 1, 1982·Journal of Toxicology and Environmental Health·J DeLucaR L Tuggy
Jul 21, 1981·Biochemistry·W P Melega, B D Howard
Jan 24, 2009·Annals of the New York Academy of Sciences·Yves DunantPaula P Gonçalves
Apr 11, 2000·Microscopy Research and Technique·Y Dunant
Oct 1, 1988·Journal of Electron Microscopy Technique·D M Landis
Nov 10, 2010·The Journal of Physiology·J Miguel CordeiroYves Dunant
Apr 24, 2013·Journal of Neurochemistry·Joao Miguel CordeiroYves Dunant
Apr 11, 2000·Microscopy Research and Technique·N Morel, M Israël
Apr 14, 1999·Journal of Neurophysiology·J H Koenig, K Ikeda
Jun 1, 1987·The American Journal of Physiology·H Rasgado-Flores, M P Blaustein
Aug 10, 2001·Fungal Genetics and Biology : FG & B·S TorralbaF P Ottensmeyer
Aug 12, 1982·Biochimica Et Biophysica Acta·N MoskowitzS Puszkin
Oct 20, 1981·Biochimica Et Biophysica Acta·G D Schellenberg, P D Swanson

❮ Previous
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