ATP stimulation of Na+/Ca2+ exchange in cardiac sarcolemmal vesicles

The American Journal of Physiology
G BerberiánL Beaugé

Abstract

In cardiac sarcolemmal vesicles, MgATP stimulates Na+/Ca2+ exchange with the following characteristics: 1) increases 10-fold the apparent affinity for cytosolic Ca2+; 2) a Michaelis constant for ATP of approximately 500 microM; 3) requires micromolar vanadate while millimolar concentrations are inhibitory; 4) not observed in the presence of 20 microM eosin alone but reinstated when vanadate is added; 5) mimicked by adenosine 5'-O-(3-thiotriphosphate), without the need for vanadate, but not by beta,gamma-methyleneadenosine 5'-triphosphate; and 6) not affected by unspecific protein alkaline phosphatase but abolished by a phosphatidylinositol-specific phospholipase C (PI-PLC). The PI-PLC effect is counteracted by phosphatidylinositol. In addition, in the absence of ATP, L-alpha-phosphatidylinositol 4,5-bisphosphate (PIP2) was able to stimulate the exchanger activity in vesicles pretreated with PI-PLC. This MgATP stimulation is not related to phosphorylation of the carrier, whereas phosphorylation appeared in the phosphoinositides, mainly PIP2, that coimmunoprecipitate with the exchanger. Vesicles incubated with MgATP and no Ca2+ show a marked synthesis of L-alpha-phosphatidylinositol 4-monophosphate (PIP) with little production of...Continue Reading

References

Jun 10, 1975·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P F Baker, H G Glitsch
Nov 1, 1992·Archives of Biochemistry and Biophysics·J F AcetoJ P Reeves
Jan 1, 1991·Annals of the New York Academy of Sciences·R DiPolo, L Beaugé
Apr 17, 1990·Biochimica Et Biophysica Acta·N Lagos, J Vergara
Apr 1, 1987·Circulation Research·R A HaworthH A Berkoff
Mar 12, 1987·Biochimica Et Biophysica Acta·R DiPolo, L Beaugé
Jun 18, 1974·Biochemical and Biophysical Research Communications·D Gratecos, E H Fischer
Oct 1, 1974·The Journal of General Physiology·R Dipolo
Aug 1, 1982·Biochemical and Biophysical Research Communications·G SwarupD L Garbers
May 16, 1983·European Journal of Biochemistry·P Caroni, E Carafoli
Jun 1, 1984·The Journal of Cell Biology·J E Ferrell, W H Huestis
Feb 21, 1980·Nature·P Caroni, E Carafoli
Apr 14, 1995·The Journal of Biological Chemistry·T IwamotoM Shigekawa
Apr 15, 1996·Annals of the New York Academy of Sciences·G Berberián, L Beaugé
Jun 7, 1996·The Journal of Biological Chemistry·T IwamotoM Shigekawa

❮ Previous
Next ❯

Citations

Oct 23, 2001·American Journal of Physiology. Heart and Circulatory Physiology·B N Eigel, R W Hadley
Mar 19, 2002·The Journal of Physiology·Reinaldo DiPolo, Luis Beaugé
May 20, 2003·The Journal of Biological Chemistry·Dan H SchulzeAbdul M Ruknudin
Dec 24, 2005·Physiological Reviews·Reinaldo DiPolo, Luis Beaugé
Dec 28, 2002·Annals of the New York Academy of Sciences·Luis BeaugéGraciela Berberián
Apr 21, 2007·Annals of the New York Academy of Sciences·Velia PosadaGraciela Berberián
May 5, 1999·The Journal of Physiology·D Kim, H Bang
Sep 16, 2006·The EMBO Journal·Michael J RiedelPeter E Light
Apr 21, 2007·Annals of the New York Academy of Sciences·Graciela BerberiánLuis Beaugé
Jun 11, 2002·American Journal of Physiology. Cell Physiology·Cem NasuhogluDonald W Hilgemann
Oct 13, 2000·American Journal of Physiology. Cell Physiology·R DiPoloL Beaugé
Sep 17, 2002·Progress in Biophysics and Molecular Biology·Reinaldo DiPolo, Luis Beaugé
Mar 20, 1999·Biochimica Et Biophysica Acta·R DiPolo, L Beaugé
Oct 12, 2010·Biochemical and Biophysical Research Communications·Diego ForcatoGraciela Berberián
Oct 4, 2011·Progress in Biophysics and Molecular Biology·Graciela BerberiánLuis Beaugé

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.