ATP synthase from bovine heart mitochondria. In vitro assembly of a stalk complex in the presence of F1-ATPase and in its absence

Journal of Molecular Biology
I R CollinsonJ E Walker


Four subunits of the F1F0-ATPase from bovine heart mitochondria have been produced by heterologous over-expression in Escherichia coli. They are the oligomycin sensitivity conferral protein (OSCP), coupling factor 6 (F6) and subunits b and d. Likewise, fragments b', bI, bC, and bM (amino acid residues 79 to 214, 121 to 214, 165 to 214 and 79 to 164, respectively, of subunit b), and fragment d' (subunit d lacking residue 1 to 14) have been produced in abundant quantities by bacterial expression. These subunits, and the fragments of subunits b and d, have been assayed singly and in various combinations by gel-filtration chromatography for their abilities to bind to bovine heart F1-ATPase. Only the OSCP was found to be capable of forming a stable binary complex with F1-ATPase. When fragments b', bI or bC were added to F1-ATPase together with the OSCP, the ternary complexes F1.OSCP.b', F1.OSCP.bI or F1.OSCP.bC were formed, but b', bI and bC appeared to be present in sub-stoichiometric amounts. When F6 was added also, then the stoichiometric quaternary complexes F1.OSCP.b'.F6 and F1.OSCP.bI.F6 were obtained, as was a fourth quaternary complex containing approximately equivalent amounts of F1 and OSCP, and sub-stoichiometric quantiti...Continue Reading


Apr 1, 2008·Journal of Biological Physics·José J García-Trejo, Edgar Morales-Ríos
Dec 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·David M ReesJohn E Walker
Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lindsay A BakerJohn L Rubinstein
Jan 25, 2011·The Journal of Biological Chemistry·Amanda K WelchBrian D Cain
Jun 7, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jocelyn A SilvesterJohn E Walker
Jan 1, 1997·Annual Review of Biochemistry·P D Boyer
Apr 26, 2007·Circulation Journal : Official Journal of the Japanese Circulation Society·San Bao ChaiChao Shu Tang
Jul 21, 2010·PLoS Biology·Praveen Balabaskaran NinaAkhil B Vaidya
Jun 23, 2006·The EMBO Journal·Veronica Kane DicksonJohn E Walker
Feb 15, 2018·Proceedings of the National Academy of Sciences of the United States of America·Jiuya HeJohn E Walker
Apr 29, 2011·Journal of Biochemistry·Daichi OkunoHiroyuki Noji
Apr 9, 2015·The Journal of Biological Chemistry·Jennifer LeeJohn E Walker
Sep 21, 2018·Frontiers in Physiology·Lilia Colina-TenorioDiego González-Halphen

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.