Abstract
Pre-translational regulation of subunit c has been suggested to control the biosynthesis of mitochondrial ATP synthase (ATPase) in brown adipose tissue (BAT). Subunit c is encoded by the genes P1 and P2, which encode identical mature proteins. We have determined here the levels of P1 and P2 mRNAs in different tissues, in response to cold acclimation in rats, during ontogenic development of BAT in hamsters, and following thyroid hormone treatment in rat BAT and liver. Quantitative ribonuclease protection analysis showed that both the P1 and P2 mRNAs were present in all rat tissues measured. Their total amount in each tissue corresponded well with the ATPase content of that tissue. While the P1/P2 mRNA ratio is high in ATPase-rich tissues, the P2 mRNA dominates in tissues with less ATPase. Cold acclimation affects P1 but not P2 gene expression in rat BAT. A rapid and transient increase in P1 mRNA is followed by sustained depression, which is accompanied by a decrease in ATPase content. Similarly, ontogenic suppression of ATPase content in hamster BAT was accompanied by suppression of the P1 mRNA levels, while P2 expression was virtually unchanged. Furthermore, when hypothyroid rats were treated with thyroid hormone, the steady-st...Continue Reading
Citations
May 3, 2008·Biological & Pharmaceutical Bulletin·Masahiro WatanabeYasuo Shinohara
Jan 29, 2011·Molecular Biology and Evolution·Michelle Déquard-ChablatVéronique Contamine
Feb 4, 2015·The Journal of Biological Chemistry·Emilia GospodarskaLeslie P Kozak
Sep 25, 1999·The Journal of Biological Chemistry·A MatthiasJ Nedergaard
Jan 13, 2004·Physiological Reviews·Barbara Cannon, Jan Nedergaard
May 8, 2001·Molecular and Cellular Biology·U Andersson, R C Scarpulla
Sep 26, 2012·Genetics and Molecular Research : GMR·W-R HouT Wang
Sep 11, 2003·FEBS Letters·Andrea PickováJosef Houstek
Sep 5, 2008·Journal of Bioenergetics and Biomembranes·Adriana Muhlia-AlmazanGloria Yepiz-Plascencia
Jan 18, 2013·PloS One·Paul E GuldeNoreen Williams
Jul 28, 2020·BMC Veterinary Research·Yuanmei WangXianyao Li
Oct 22, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Shiro ItoiShugo Watabe
Nov 18, 2000·European Journal of Biochemistry·T HimedaT Higuti
Aug 27, 2016·The Journal of Biological Chemistry·Callie P WigingtonAnita H Corbett
Aug 2, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tatiana V KramarovaBarbara Cannon
Dec 29, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Hela BahriStéphane Duvezin-Caubet
Oct 15, 2020·ELife·Neel S SinghalDengke K Ma
Feb 13, 2001·Gene·Y ChinenovM E Martin
Mar 10, 2001·Biochimica Et Biophysica Acta·J NedergaardB Cannon
Jul 30, 2004·Biochimica Et Biophysica Acta·Josef HoustekJirí Zeman
Feb 8, 2006·Gene·Anna De GrassiCecilia Saccone
May 30, 2006·Biochimica Et Biophysica Acta·Josef HoustekPavel Jesina
Oct 7, 2006·Medical Hypotheses·Neville Grant
Jul 16, 2008·Biochimica Et Biophysica Acta·Roza KucharczykJean-Paul di Rago