ATP11C Facilitates Phospholipid Translocation across the Plasma Membrane of All Leukocytes

PloS One
Mehmet YabasAnselm Enders

Abstract

Organization of the plasma membrane into specialized substructures in different blood lineages facilitates important biological functions including proper localization of receptors at the plasma membrane as well as the initiation of crucial intracellular signaling cascades. The eukaryotic plasma membrane is a lipid bilayer that consists of asymmetrically distributed phospholipids. This asymmetry is actively maintained by membrane-embedded lipid transporters, but there is only limited data available about the molecular identity of the predominantly active transporters and their substrate specificity in different leukocyte subsets. We demonstrate here that the P4-type ATPase ATP11C mediates significant flippase activity in all murine leukocyte subsets. Loss of ATP11C resulted in a defective internalization of phosphatidylserine (PS) and phosphatidylethanolamine (PE) in comparison to control cells. The diminished flippase activity caused increased PS exposure on 7-aminoactinomycin D- (7-AAD-) viable pro-B cells freshly isolated from the bone marrow of ATP11C-deficient mice, which was corrected upon a 2-hour resting period in vitro. Despite the impaired flippase activity in all immune cell subsets, the only other blood cell type wi...Continue Reading

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Citations

Oct 26, 2018·Proceedings of the National Academy of Sciences of the United States of America·Katsumori SegawaShigekazu Nagata
Dec 5, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hye-Won Shin, Hiroyuki Takatsu
Jan 25, 2019·Blood Advances·Weidong JingStefan Bröer
Jul 28, 2016·Frontiers in Physiology·Jens P AndersenRobert S Molday
Jul 3, 2020·Cell Death & Disease·Jee-Hae KangSun-Hun Kim
Oct 13, 2020·The Biochemical Journal·Rosa L López-MarquésMichael Palmgren
Mar 14, 2021·Cellular and Molecular Life Sciences : CMLS·Merryn FraserAlexander G Maier

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Methods Mentioned

BETA
Assay
flow cytometry

Software Mentioned

GraphPad Prism

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