ATP2C1 is specifically localized in the basal layer of normal epidermis and its depletion triggers keratinocyte differentiation

Journal of Dermatological Science
Masaki YoshidaHiroshi Suzuki

Abstract

ATP2C1 is a calcium/manganese-ATPase localized in the Golgi apparatus and known as responsible gene for Hailey-Hailey disease. But its localization and roles in the epidermis are not fully elucidated. To explore the localization and biological role of ATP2C1 in normal epidermis in terms of differentiation states. We examined the immunohistochemical distribution of ATP2C1 in normal epidermis and measured the expression of ATP2C1 in cultured keratinocytes following forced detachment from culture dish or following treatment with high concentrations of calcium. Furthermore, we knockdown ATP2C1 expression in cultured keratinocytes by using RNA interference procedure to abrogate cation accumulation in cell organelles. ATP2C1 is specifically localized at the basal cell layer in normal epidermis. Neither detachment of keratinocyte from culture dish nor treatment with high concentrations of calcium suppressed ATP2C1 expression, while both procedures induced differentiation markers, K10 keratin and involucrin. In contrast, knockdown of ATP2C1 induced these differentiation markers of cultured keratinocytes. Furthermore, treatment of keratinocytes with a calcium ionophore, A23187, did not up-regulate differentiation markers of keratinocyte...Continue Reading

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Citations

Feb 2, 2010·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hiroshi SuzukiStefania Danko
Dec 5, 2006·Cell Calcium·Ludwig MissiaenFrank Wuytack
Oct 1, 2009·Physiological Reviews·Marisa Brini, Ernesto Carafoli
Feb 13, 2014·The Cochrane Database of Systematic Reviews·Elizabeth McGinnis, Nikki Stubbs
Feb 26, 2015·The Journal of Biological Chemistry·Néstor García-RodríguezRalf Erik Wellinger
Oct 26, 2018·International Journal of Health Care Quality Assurance·Dinesh Kumar

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