Atp6v0d2 is an essential component of the osteoclast-specific proton pump that mediates extracellular acidification in bone resorption.

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Haiping WuYi-Ping Li

Abstract

Bone resorption relies on the extracellular acidification function of vacuolar (V-) ATPase proton pump(s) present in the plasma membrane of osteoclasts. The exact configuration of osteoclast-specific V-ATPases remains largely unknown. In this study, we found that Atp6v0d2 (d2), an isoform of the d subunit in the V-ATPase, showed 5-fold higher expression than that of Atp6v0d1 (d1) in mature osteoclasts, indicating a potential function in osteoclastic bone resorption. When d2 was depleted at an early stage of RANKL-induced osteoclast differentiation in vitro, formation of multinucleated cells was severely impaired. However, depletion of d2 at a late differentiation stage did not affect osteoclast fusion but did abolish the activity of extracellular acidification and bone resorption of mature osteoclasts. We also showed the association of the two tagged-proteins d2 and a3 when co-expressed in mammalian cells with a co-immunoprecipitation assay. Moreover, glutathione-S-transferase (GST) pull-down assay showed the direct interaction of d2 with the N terminus of Atp6v0a3 (a3), which is the functionally identified osteoclast-specific component of V-ATPase. Therefore, our results show the dual function of d2 as a regulator of cell fusi...Continue Reading

Citations

Jan 1, 2009·Journal of Innate Immunity·Mark T Quinn, Igor A Schepetkin
Jul 3, 2010·Calcified Tissue International·Jonas K E Nyman, H Kalervo Väänänen
May 28, 2010·Kidney International·Gabin SihnMichael Bader
Jun 2, 2012·The International Journal of Biochemistry & Cell Biology·A QinM H Zheng
Oct 25, 2011·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Yusheng YaoChunming Liu
Mar 23, 2011·Gastroenterología y hepatología·Miguel Martí-CabreraJuan V Esplugues
Jun 25, 2010·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Ana Paula Oliveira GiorgettiFrancisco Humberto Nociti
Oct 13, 2009·Biochemical and Biophysical Research Communications·Taesoo KimYongwon Choi
Apr 3, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·De-Qin YangYi-Ping Li
Nov 7, 2012·Journal of Cellular Biochemistry·Gazelle J CrastoMorris F Manolson
Aug 5, 2009·Journal of Cellular Physiology·Myung Hee KimSeong Hwan Kim
Sep 13, 2015·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xiang ZhuTak S Cheng
Feb 25, 2014·Biochimica Et Biophysica Acta·Naomi MatsumotoMayumi Nakanishi-Matsui
Aug 24, 2013·Cell Cycle·Julie LacombeMathieu Ferron
Apr 19, 2015·Arthritis Research & Therapy·Katja C M DenningerÅsa Andersson
Aug 9, 2016·Environmental Toxicology and Pharmacology·Pei JunruiSun Dianjun
Mar 22, 2015·Journal of Dental Research·H KanzakiY Nakamura
Mar 16, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Motoya MuraseTaro Kawai
Mar 14, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Takenobu IshiiToshihisa Kawai
Sep 15, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jiae LeeWoojin Jeong
Feb 12, 2019·Free Radical Research·Hirofumi FujitaHideyo Ohuchi
May 6, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kangxian ZhaoYu Qian
Dec 10, 2015·International Journal of Molecular Medicine·Shuangxin LiuWei Shi
May 5, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Katharina JähnLynda F Bonewald
Oct 31, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Danielle TokarzEva S Liu
May 2, 2018·Scientific Reports·Naomi MatsumotoMayumi Nakanishi-Matsui
Nov 16, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fuying ChenXingdong Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.