ATPase kinetics on activation of rabbit and frog permeabilized isometric muscle fibres: a real time phosphate assay

The Journal of Physiology
Z HeM A Ferenczi

Abstract

1. The rate of appearance of inorganic phosphate (Pi) and hence the ATPase activity of rabbit psoas muscle in single permeabilized muscle fibres initially in rigor was measured following laser flash photolysis of the P3-1-(2-nitrophenyl)ethyl ester of ATP (NPE-caged ATP) in the presence and absence of Ca2+. Pi appearance was monitored from the fluorescence signal of a Pi-sensitive probe, MDCC-PBP, a coumarin-labelled A197C mutant of the phosphate-binding protein from Escherichia coli. Fibres were immersed in oil to optimize the fluorescence signal and to obviate diffusion problems. The ATPase activity was also measured under similar conditions from the rate of NADH disappearance using an NADH-linked coupled enzyme assay. 2. On photolysis of NPE-caged ATP in the presence of Ca2+ at 20 degrees C, the fluorescence increase of MDCC-PBP was non-linear with time. ATPase activity was 41 s-1 in the first turnover based on a myosin subfragment 1 concentration of 150 microM. This was calculated from a linear regression of the fluorescence signal reporting 20-150 microM of Pi release. Tension was at 67% of its isometric level by the time 150 microM Pi was released. ATPase activities were 36 and 31 s-1 for Pi released in the ranges of 150-...Continue Reading

References

Jul 1, 1975·The Journal of General Physiology·B A Mobley, B R Eisenberg
Jan 1, 1989·Methods in Enzymology·J W WalkerD R Trentham
Jan 1, 1986·Annual Review of Biophysics and Biophysical Chemistry·M G Hibberd, D R Trentham
Jan 1, 1987·Annual Review of Physiology·Y E Goldman
Jun 14, 1985·Science·M G HibberdY E Goldman
Sep 1, 1986·Biophysical Journal·M A Ferenczi
Nov 1, 1985·Biophysical Journal·R Cooke, E Pate
Jun 20, 1969·Science·H E Huxley
Jan 1, 1968·Pflügers Archiv : European journal of physiology·H G Mannherz
May 30, 1973·Biochimica Et Biophysica Acta·S Marston
Dec 30, 1972·Journal of Molecular Biology·I Matsubara, G F Elliott
Oct 22, 1971·Nature·A F Huxley, R M Simmons
Feb 15, 1984·Journal of Molecular Biology·I MatsubaraR M Simmons
Jul 1, 1984·The Journal of Physiology·M A FerencziD R Trentham
Jul 25, 1983·Journal of Molecular Biology·L D Yates, M L Greaser
May 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Cooke
Nov 1, 1994·The Journal of Cell Biology·K HiroseJ M Murray
May 1, 1994·Biophysical Journal·Y Z Ma, E W Taylor
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·G C Ellis-Davies, J H Kaplan
Sep 15, 1995·The Journal of Physiology·M Linari, R C Woledge

❮ Previous
Next ❯

Citations

May 29, 2008·Annals of Biomedical Engineering·D A Smith, S M Mijailovich
Aug 26, 2000·Progress in Biophysics and Molecular Biology·R Bottinelli, C Reggiani
Nov 14, 1997·International Journal of Biological Macromolecules·G F Elliott, C R Worthington
Apr 1, 2000·Biochimica Et Biophysica Acta·J M Morel, N D'Hahan
Apr 20, 2012·The Journal of Biological Chemistry·David PoppRobert C Robinson
Jun 14, 2012·The Journal of Biological Chemistry·Catherine MansfieldMichael A Ferenczi
Oct 17, 2003·The Journal of Physiology·Giuseppe D'AntonaRoberto Bottinelli
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·L C RomeY E Goldman
Mar 29, 2008·The Journal of Physiology·Alison K StubbingsMichael A Ferenczi
Oct 21, 2003·The Journal of Physiology·Timothy G WestRoger C Woledge
Mar 4, 2006·Biophysical Journal·V B SiththanandanM A Ferenczi
Nov 4, 2009·Scandinavian Journal of Medicine & Science in Sports·E BorinaR Bottinelli
Apr 2, 2003·Biophysical Journal·J Borejdo, I Akopova
May 12, 2015·BioMed Research International·Alf MånssonGeorgios Tsiavaliaris
Apr 17, 2016·European Biophysics Journal : EBJ·Haiyang YuMichael A Ferenczi
Jan 30, 2004·Biophysical Journal·Hunter MartinRobert J Barsotti
Jul 18, 2006·Journal of Theoretical Biology·Enrico Grazi, Cristina Di Bona
Aug 16, 2005·Biophysical Chemistry·Enrico Grazi, Cristina Di Bona
Mar 31, 2009·International Journal of Molecular Sciences·Robert Jarosch
May 21, 1998·Nature Biotechnology·T Fernandez, H Bayley
Feb 5, 2005·Journal of Applied Physiology·Jason J HamannPhilip S Clifford

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.