ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure

Cell
Saumya JainRoy Parker

Abstract

Stress granules are mRNA-protein granules that form when translation initiation is limited, and they are related to pathological granules in various neurodegenerative diseases. Super-resolution microscopy reveals stable substructures, referred to as cores, within stress granules that can be purified. Proteomic analysis of stress granule cores reveals a dense network of protein-protein interactions and links between stress granules and human diseases and identifies ATP-dependent helicases and protein remodelers as conserved stress granule components. ATP is required for stress granule assembly and dynamics. Moreover, multiple ATP-driven machines affect stress granules differently, with the CCT complex inhibiting stress granule assembly, while the MCM and RVB complexes promote stress granule persistence. Our observations suggest that stress granules contain a stable core structure surrounded by a dynamic shell with assembly, disassembly, and transitions between the core and shell modulated by numerous protein and RNA remodeling complexes.

References

May 29, 2002·The EMBO Journal·Katharina Ribbeck, Dirk Görlich
Jul 31, 2003·Journal of Molecular Biology·Magali Frugier, Richard Giegé
Sep 17, 2004·Molecular Biology of the Cell·Natalie GilksPaul Anderson
Jun 22, 2005·The Journal of Cell Biology·Nancy KedershaPaul Anderson
Jul 11, 2006·The Journal of Biological Chemistry·Jae-Ho Shin, Zvi Kelman
Jan 25, 2007·Cell Cycle·Chi Kwan Tsang, X F Steven Zheng
May 4, 2007·Molecular Biology of the Cell·Muriel Brengues, Roy Parker
Jun 11, 2008·Optics Express·Sri Rama Prasanna Pavani, Rafael Piestun
Jul 10, 2008·Journal of Cell Science·Martin A M ReijnsJean D Beggs
Nov 5, 2008·The Journal of Cell Biology·J Ross BuchanRoy Parker
Feb 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sri Rama Prasanna PavaniW E Moerner
Oct 15, 2009·Journal of Cell Science·Mariela LoschiGraciela L Boccaccio
Jan 13, 2010·Molecular Cell·J Ross Buchan, Roy Parker
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Clifford P BrangwynneAnthony A Hyman
Sep 20, 2011·Biochimica Et Biophysica Acta·Yoshito Kakihara, Walid A Houry
Oct 27, 2011·Journal of Neurology, Neurosurgery, and Psychiatry·Alexander M RossorMary M Reilly
Mar 20, 2012·Cell·David Eisenberg, Mathias Jucker
Apr 17, 2012·Structure·Alexander LeitnerJudith Frydman
Jun 5, 2012·Cell·Alfredo CastelloMatthias W Hentze
Jun 12, 2012·Cell·Stephanie C Weber, Clifford P Brangwynne
Apr 10, 2013·Human Mutation·Hélène BlascoUNKNOWN French ALS Study Group
May 1, 2013·The Journal of Cell Biology·Yun R LiAaron D Gitler
Aug 21, 2013·Cell·Mani RamaswamiRoy Parker
Sep 13, 2014·Science·John Rinn, Mitchell Guttman
Nov 7, 2014·PLoS Genetics·Xiaoxue YangPer Sunnerhagen
May 28, 2015·Proceedings of the National Academy of Sciences of the United States of America·Shana Elbaum-GarfinkleClifford P Brangwynne
Aug 6, 2015·Nature Communications·Tatjana TrcekRuth Lehmann

❮ Previous
Next ❯

Citations

Mar 22, 2016·Viruses·Renée L Finnen, Bruce W Banfield
Apr 2, 2016·Current Genetics·Juha Saarikangas, Yves Barral
Apr 8, 2016·Trends in Cell Biology·Adriano Aguzzi, Matthias Altmeyer
Apr 7, 2016·Cell Communication and Signaling : CCS·Aino I JärvelinAlfredo Castello
Apr 24, 2016·Nucleic Acids Research·Brianne E DocterJames C A Bardwell
Apr 20, 2016·Methods : a Companion to Methods in Enzymology·Boris L ZybailovKevin D Raney
May 21, 2016·Cell·Louis-Philippe Bergeron-SandovalStephen W Michnick
May 24, 2016·Cell·Marina FericClifford P Brangwynne
Jun 4, 2016·Current Opinion in Structural Biology·Randal Halfmann
Jun 9, 2016·Acta Neuropathologica·Steven BoeynaemsLudo Van Den Bosch
Jun 13, 2016·Trends in Cell Biology·David S W Protter, Roy Parker
Jul 2, 2016·Viruses·Natalia Poblete-DuránFernando Valiente-Echeverría
Jul 5, 2016·Cell·Salman F BananiMichael K Rosen
Aug 10, 2016·Wiley Interdisciplinary Reviews. RNA·Domenica MarcheseGian G Tartaglia
Aug 11, 2016·Nucleic Acids Research·Christina A ViveloAnthony K L Leung
Aug 25, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Simon Alberti, Anthony A Hyman
Sep 8, 2016·ELife·Joshua R WheelerRoy Parker
Oct 30, 2016·RNA Biology·Scott Horowitz, James C A Bardwell
Nov 5, 2016·ELife·Christopher Frederick MuglerKarsten Weis
Nov 8, 2016·Trends in Genetics : TIG·Rico SchieweckMichael A Kiebler
Nov 9, 2016·The Journal of Cell Biology·Marc D PanasPaul Anderson
Oct 14, 2016·Molecular Biology of the Cell·Dipen RajgorCatherine M Shanahan
Jan 14, 2017·Cell·Giulia Paci, Edward A Lemke
Feb 23, 2017·Nature Reviews. Molecular Cell Biology·Salman F BananiMichael K Rosen
Jan 26, 2017·ACS Nano·Alexander V RomashchenkoYuri M Moshkin
Mar 18, 2017·Molecular Cell·Steven BoeynaemsLudo Van Den Bosch
May 11, 2017·Annual Review of Biochemistry·Emily Mitchell SontagJudith Frydman
Jun 29, 2017·Angewandte Chemie·Nan-Nan Deng, Wilhelm T S Huck
Feb 28, 2018·Proceedings of the National Academy of Sciences of the United States of America·Briana Van TreeckRoy Parker
Feb 9, 2018·Journal of Neural Transmission·S BräuerA Hermann
Feb 22, 2018·Antioxidants & Redox Signaling·Karl-Josef DietzThorsten Seidel
Jan 25, 2018·The Journal of Cell Biology·Tomas GrouslAxel Mogk
Feb 22, 2018·The Journal of Cell Biology·Benedikt NiewidokRoland Brandt
Jan 3, 2018·The Journal of Pathology·Amal M El-Naggar, Poul H Sorensen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.