A simple procedure for the purification of Mg2+-stimulated ATPase of Escherichia coli by fractionation with poly(ethylene glycols) and gel filtration is described. The enzyme restores ATPase-linked reactions to membrane preparations lacking these activities. Five different polypeptides (alpha, beta, gamma, delta, epsilon) are observed in sodium dodecyl sulfate electrophoresis. Freezing in salt solutions splits the enzyme complex into subunits which do not possess any catalytic activity. The presence of different subunits is confirmed by electrophoretic and immunological methods. The active enzyme complex can be reconstituted by decreasing the ionic strength in the dissociated sample. Temperature, pH, protein concentration, and the presence of substrate are each important determinants of the rate and extent of reconstitution. The dissociated enzyme has been separated by ion-exchange chromatography into two major fragments. Fragment IA has a molecular weight of about 100000 and contains the alpha, gamma, and epsilon polypeptides. The minor fragment, IB, has about the same molecular weight but contains, besides alpha, gamma, and epsilon, the delta polypeptide. Fragment II, with a molecular weight of about 52000, appears to be iden...Continue Reading
Mitochondrial adenosine triphosphatase. Location of sulfhydryl groups and disulfide bonds in soluble enzyme from beef heart
Subunit composition, function, and spatial arrangement in the Ca2+-and Mg2+-activated adenosine triphosphatases of Escherichia coli and Salmonella typhimurium
Membrane reconstitution in chl-r mutants of Escherichia coli K 12. VIII. Purification and properties of the FA factor, the product of the chl B gene
Energy conservation in membranes of mutants of Escherichia coli defective in oxidative phosphorylation
Purification and properties of reconstitutively active and inactive adenosinetriphosphatase from Escherichia coli
Properties of a soluble Ca 2+ - and Mg 2+ -activated ATPase released from Escherichia coli membranes
Membrane ATPase of Escherichia coli K 12. Selective solubilization of the enzyme and its stimulation by trypsin in the soluble and membrane-bound states
Oxidative phosphorylation and proton translocation in membrane vesicles prepared from Escherichia coli
Isolation and purification of bacterial membrane proteins by the use of organic solvents: the lactose permease and the carbodiimide-reactive protein of the adenosinetriphosphatase complex of Escherichia coli
The nucleotide sequence of the atp genes coding for the F0 subunits a, b, c and the F1 subunit delta of the membrane bound ATP synthase of Escherichia coli
Complementation of a Chlamydomonas reinhardtii mutant defective in the nuclear gene encoding the chloroplast coupling factor 1 (CF1) gamma-subunit (atpC)
Evidence supporting the identity of beef heart mitochondrial chloroform-released adenosine triphosphatase (ATPase) with coupling factor I
Novel approaches towards characterization of the high-affinity nucleotide binding sites on mitochondrial F1-ATPase by the fluorescence probes 3'-O-(1-naphthoyl)adenosine di- and triphosphate
Characterization of the ATPase activity of the N-terminal nucleotide binding domain of an ABC transporter involved in oleandomycin secretion by Streptomyces antibioticus
The dicyclohexylcarbodiimide-binding protein c of ATP synthase from Escherichia coli is not sufficient to express an efficient H+ conduction
The WaaL O-antigen lipopolysaccharide ligase has features in common with metal ion-independent inverting glycosyltransferases
The atp operon: nucleotide sequence of the region encoding the alpha-subunit of Escherichia coli ATP-synthase
ATPase activity and ATP/ADP-induced conformational change in the soluble domain of the bacterial protein translocator HlyB
The use of several energy-coupling reactions in characterizing mutants of Escherichia coli K12 defective in oxidative phosphorylation
A mutant ATP synthetase of Escherichia coli with an altered sensitivity to N,N' -dicyclohexylcarbodiimide: characterization in native membranes and reconstituted proteoliposomes
The ATP synthetase of Escherichia coli K12: purification of the enzyme and reconstitution of energy-transducing activities
Size and shape of the alpha subunit of the (Ca,Mg)-dependent adenosinetriphosphate from Escherichia coli in solution in the presence and absence of ATP
ATP synthetase (F1F0) of Escherichia coli K-12. High-yield preparation of functional F0 by hydrophobic affinity chromatography
Biosynthesis of ascorbate in yeast. Purification of L-galactono-1,4-lactone oxidase with properties different from mammalian L-gulonolactone oxidase
Chemical modification of the F0 part of the ATP synthase (F1F0) from Escherichia coli. Effects on proton conduction and F1 binding
Azidonaphthoyl-ADP: a specific photolabel for the high-affinity nucleotide-binding sites of F1-ATPase
Total number and differentiation of nucleotide binding sites on mitochondrial F1-ATPase. An approach by photolabeling and equilibrium binding studies
Accessibility of F0 subunits from Escherichia coli ATP synthase. A study with subunit specific antisera
Characterization of the Na+-stimulated ATPase of Propionigenium modestum as an enzyme of the F1F0 type
Cross-reconstitution of the F0F1-ATP synthases of chloroplasts and Escherichia coli with special emphasis on subunit delta
Purification and characterization of the F1 portion of the ATP synthase (F1Fo) of Streptomyces lividans
Structure of beef heart mitochondrial F1-ATPase. Arrangement of subunits as disclosed by cross-linking reagents and selective labeling by radioactive ligands
Demonstration of two exchangeable non-catalytic and two cooperative catalytic sites in isolated bovine heart mitochondrial F1, using the photoaffinity labels [2-3H]8-azido-ATP and [2-3H]8-azido-ADP
Extrinsic and intrinsic factors that influence inactivation and purification of the unstable adenosine triphosphatase solubilized from membranes of an Escherichia coli K 12 strain
Purification of the DCCD-reactive protein of the energy-transducing adenosine triphosphatase complex from Escherichia coli
Effect of ATP on the translational diffusion coefficient of the alpha-subunit of Escherichia coli F1-ATPase
The mitochondrial ATPase of brown adipose tissue. Purification and comparison with the mitochondrial ATPase from beef heart
The isolated F0 of Escherichia coli aTP-synthase is reconstitutively active in H+-conduction and ATP-dependent energy-transduction
F0 of Escherichia coli ATP-synthase containing mutant and wild-type carbodiimide-binging proteins is impaired in H+ -conduction
Physical characterization of the reconstituted F1-ATPase of Escherichia coli from the main subunits (alpha, beta, gamma)
Specific interaction of the new fluorescent dye 10-N-nonyl acridine orange with inner mitochondrial membrane. A lipid-mediated inhibition of oxidative phosphorylation
The binding of aurovertin to isolated beta subunit of F1 (mitochondrial ATPase). Stoicheiometry of beta subunit in F1
Use of monoclonal antibodies in immuno-electron microscopy for the determination of subunit stoichiometry in oligomeric enzymes. There are three alpha-subunits in the F1-ATPase of Escherichia coli
Sulfhydryl groups of the F1 adenosine triphosphatase of Escherichia coli and the stoichiometry of the subunits
Orientation of subunit c of the ATP synthase of Escherichia coli--a study with peptide-specific antibodies
Limited proteolysis of coupling factor-latent ATPase from Mycobacterium phlei. Effects of different enzymes and modifying agents
1-fluoro-2, 4-dinitrobenzene modifies a tyrosine residue when it inactivates the bovine mitochondrial F(1)-ATPase
Hysteretic properties of soluble F1 ATPase from Escherichia coli. Evidence for a slow transition between two conformational states of the enzyme
Sequence of the radioactive tryptic peptide obtained after inactivating the F1-ATPase of the thermophilic bacterium PS3 with 5'-p-fluorosulfonylbenzoyl[3H]adenosine at 65 degrees C
The use of [3H]aniline to identify the essential carboxyl group in the bovine mitochondrial F1-ATPase that reacts with 1-(ethoxycarbonyl)-2-ethoxy-1,2-dihydroquinoline
Kinetic modelling of the Zymomonas mobilis Entner-Doudoroff pathway: insights into control and functionality
In vivo and in vitro incorporation of endogenous nucleotides by the energy-transducing ATPase of Streptococcus faecalis
Subunit specific antisera to the Escherichia coli ATP synthase: effects on ATPase activity, energy transduction, and enzyme assembly
Stabilization of rat liver mitochondrial F1-adenosine triphosphatase during chloroform-induced solubilization
Reconstitution of ATPase activity from the isolated alpha, beta, and gamma subunits of the coupling factor, F1, of Escherichia coli
An antibody-binding site in the native enzyme between amino acid residues 205-287 of the gamma-subunit of F1 from Escherichia coli
Conversion of stable ATPase to labile ATPase by acetylation, and the alpha beta and alpha gamma subunit complexes during its reconstitution
Topological and functional aspects of the proton conductor, F0, of the Escherichia coli ATP-synthase
Inhibition of an oligomycin-sensitive ATPase by cationic dyes, some of which are atypical uncouplers of intact mitochondria
Chymotryptic conversion of bacterial membrane ATPase to an active form with modified alpha chains and defective membrane binding properties
Characterization of the purified membrane attachment (beta) subunit of the proton translocating adenosine triphosphatase from Escherichia coli
Inactivation of Escherichia coli BF1-ATPase by dicyclohexylcarbodiimide. Chemical modification of the beta subunit
Molecular properties of random coil and refolded forms of alpha and beta subunits of an energy transducing ATPase from bacterial membranes
Reactivity of the beta subunit of Escherichia coli adenosine triphosphatase with 4-chloro-7-nitrobenzofurazan
Exploration of adenosine 5'-diphosphate-adenosine 5'-triphosphate binding sites of Escherichia coli adenosine 5'-triphosphatase with arylazido adenine nucleotides
Chemical modification of F1-ATPase by dicyclohexylcarbodiimide: application to analysis of the stoichiometry of subunits in Escherichia coli F1
Reaction of the ArsA adenosinetriphosphatase with 2-(4'-maleimidoanilino)naphthalene-6-sulfonic acid
F1-ATPase from Micrococcus sp. ATCC 398. Purification by ion-exchange chromatography and further characterization. (Auto)proteolysis and dissociative effects
Hydrogen formation from glycolate driven by reversed electron transport in membrane vesicles of a syntrophic glycolate-oxidizing bacterium
The ATP hydrolytic activity of purified ZntA, a Pb(II)/Cd(II)/Zn(II)-translocating ATPase from Escherichia coli
ArsD residues Cys12, Cys13, and Cys18 form an As(III)-binding site required for arsenic metallochaperone activity
Electron transport phosphorylation driven by glyoxylate respiration with hydrogen as electron donor in membrane vesicles of a glyoxylate-fermenting bacterium
Enzymatic characterization of FliI. An ATPase involved in flagellar assembly in Salmonella typhimurium.
P-glycoprotein is stably inhibited by vanadate-induced trapping of nucleotide at a single catalytic site.
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