PMID: 7540110Mar 1, 1995Paper

ATPo but not cAMPi activates a chloride conductance in mouse ventricular myocytes

Cardiovascular Research
P C Levesque, J R Hume

Abstract

The aim was to test for the presence of cAMPi activated and ATPo activated chloride conductances (ICl(cAMP) and ICl(ATP)) in mouse ventricular myocytes. Membrane currents were measured in enzymatically dissociated ventricular myocytes using the whole cell configuration of the patch clamp technique. After inhibiting the activation of sodium, calcium, and potassium currents, increasing [cAMP]i with isoprenaline (1 microM), forskolin (10 microM), or isobutylmethylxanthine (500 microM) had no effect on residual membrane current but extracellular ATP (100 microM) elicited a time independent background conductance. The ATP-activated conductance was observed during voltage steps ranging from +40 to -110 mV from a holding potential of -30 mV within 1 min after adding ATP to the bath. The reversal potential for the ATP activated conductance varied with changes in the chloride gradient and was within several mV of the predicted Nernst potential for chloride. Partially substituting external chloride with aspartate attenuated the ATP activated currents and shifted the reversal potential to values close to those predicted for ECl, suggesting the chloride selective nature of the conductance. The poorly hydrolysable ATP analogue ATP gamma S (...Continue Reading

Citations

Feb 29, 2000·British Journal of Pharmacology·C I SpencerR Z Kozlowski
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·P HartJ R Hume
Jan 26, 2006·The Journal of Biological Chemistry·Xue-Qian ZhangJoseph Y Cheung
Mar 30, 2010·Acta Physiologica·G BurnstockA Verkhratsky
Feb 24, 2004·The Journal of Physiology·Shintaro Yamamoto-MizumaJoseph R Hume
Dec 13, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Shintaro Yamamoto, Tsuguhisa Ehara
Feb 4, 2014·American Journal of Physiology. Heart and Circulatory Physiology·JuFang WangJoseph Y Cheung

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