PMID: 19146767Jan 17, 2009Paper

ATR, BRCA1 and gammaH2AX localize to unsynapsed chromosomes at the pachytene stage in human oocytes

Reproductive Biomedicine Online
R Garcia-CruzM Garcia Caldés

Abstract

Asynapsis of homologous chromosomes at the pachytene stage has been associated with gametogenic failure and infertility, but the cellular mechanisms involved are currently unknown in human meiocytes. In mice, the protein encoded by the breast-cancer susceptibility gene Brca1 has been described to direct kinase ATR (ataxia telangiectasia and Rad3 related) to any unpaired DNA at the pachytene stage, where ATR triggers H2AX phosphorylation, resulting in the silencing of those chromosomes. In this study, the distribution of ATR, BRCA1 and the phosphorylated histone gammaH2AX is assessed by immunofluorescence in human oocytes and it is found that they localize at unpaired chromosomes at the pachytene stage. Evidence is shown to propose that BRCA1, ATR and gammaH2AX in the human may be part of a system such as the one previously described in mouse, which signals unsynapsed chromosomes at pachytene and may lead to their silencing.

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Citations

Jul 7, 2011·Cold Spring Harbor Perspectives in Biology·Edith Heard, James Turner
Dec 10, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Kutluk OktaySamir N Babayev
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Jun 18, 2009·Journal of Cell Science·Anna KouznetsovaChrister Höög
Jun 1, 2021·Chemico-biological Interactions·Sharmistha ChoudhuriShailendra Asthana

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