ATR-FTIR Microspectroscopy Brings a Novel Insight Into the Study of Cell Wall Chemistry at the Cellular Level

Frontiers in Plant Science
Clément CuelloAnnabelle Déjardin

Abstract

Wood is a complex tissue that fulfills three major functions in trees: water conduction, mechanical support and nutrient storage. In Angiosperm trees, vessels, fibers and parenchyma rays are respectively assigned to these functions. Cell wall composition and structure strongly varies according to cell type, developmental stages and environmental conditions. This complexity can therefore hinder the study of the molecular mechanisms of wood formation, underlying the construction of its properties. However, this can be circumvented thanks to the development of cell-specific approaches and microphenotyping. Here, we present a non-destructive microphenotyping method based on attenuated total reflectance-Fourier transformed infrared (ATR-FTIR) microspectroscopy. We applied this technique to three types of poplar wood: normal wood of staked trees (NW), tension and opposite wood of artificially tilted trees (TW, OW). TW is produced by angiosperm trees in response to mechanical strains and is characterized by the presence of G fibers, exhibiting a thick gelatinous extra-layer, named G-layer, located in place of the usual S2 and/or S3 layers. By contrast, OW located on the opposite side of the trunk is totally deprived of fibers with G-l...Continue Reading

References

Jan 9, 1999·The Plant Journal : for Cell and Molecular Biology·L ChenM C McCann
Jul 31, 2003·The Plant Journal : for Cell and Molecular Biology·Grégory MouilleHerman Höfte
Jun 12, 2004·Biological Reviews of the Cambridge Philosophical Society·J R Barnett, Victoria A Bonham
Dec 14, 2004·Comptes rendus biologies·Gilles PilateKatia Ruel
Feb 21, 2006·Plant Physiology·Notburga Gierlinger, Manfred Schwanninger
Apr 25, 2008·Current Opinion in Plant Biology·Ewa J Mellerowicz, Björn Sundberg
Jul 19, 2008·Biomacromolecules·Notburga GierlingerManfred Schwanninger
Jan 30, 2009·The Plant Journal : for Cell and Molecular Biology·Charleen L Courtois-MoreauHannele Tuominen
Apr 8, 2010·Comptes rendus biologies·Annabelle DéjardinJean-Charles Leplé
May 18, 2010·Plant Physiology·Frederik R D van ParijsRoeland M H Merks
Oct 31, 2014·Applied Spectroscopy·Richard ChazalFabienne Guillon
Jul 31, 2018·Applied Spectroscopy Reviews·Notburga Gierlinger
Oct 1, 2004·The New Phytologist·Florian LafarguetteGilles Pilate
Oct 1, 2004·The New Phytologist·Gilles PilateJean-Charles Leplé

❮ Previous
Next ❯

Methods Mentioned

BETA
environmental stresses
PCA
atomic force microscopy
chemical treatments

Software Mentioned

gplots
prospectr
R stats package
SpectrumImage
quilt
FactoMineR
plot
R

Related Concepts