ATR-FTIR redox difference spectroscopy of Yarrowia lipolytica and bovine complex I.

Biochemistry
Douglas MarshallPeter R Rich

Abstract

ATR-FTIR spectroscopy in combination with electrochemistry has been applied to the redox centers of Yarrowia lipolytica complex I. The redox spectra show broad similarities with previously published data on Escherichia coli complex I and with new data here on bovine complex I. The spectra are dominated by amide I/II protein backbone changes. Comparisons with redox IR spectra of small model ferredoxins demonstrate that these amide I/II changes arise primarily from characteristic structural changes local to the iron-sulfur centers, rather than from global structural alterations as has been suggested previously. Bands arising from the substrate ubiquinone were evident, as was a characteristic 1405 cm(-)(1) band of the reduced form of the FMN cofactor. Other signals are likely to arise from perturbations or protonation changes of a carboxylic amino acid, histidine, and possibly several other specific amino acids. Redox difference spectra of center N2, together with substrate ubiquinone, were isolated from those of the other iron-sulfur centers by selective redox potentiometry. Its redox-linked amide I/II changes were typical of those in other 4Fe-4S iron sulfur proteins. Contrary to published data on bacterial complex I, no center ...Continue Reading

References

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Citations

Sep 1, 2007·Biochemistry·Vera G GrivennikovaAndrei D Vinogradov
May 30, 2007·Molecular BioSystems·Peter R Rich, Masayo Iwaki
Jul 16, 2014·Biochimica Et Biophysica Acta·Petra Hellwig
Dec 8, 2007·Analytical Biochemistry·Frédéric FournierDavid R Klug
Jul 7, 2015·Chembiochem : a European Journal of Chemical Biology·Sébastien KriegelPetra Hellwig
Dec 23, 2009·The Biochemical Journal·Judy Hirst
Nov 13, 2013·Biochimica Et Biophysica Acta·Marina Verkhovskaya, Mårten Wikström
Sep 18, 2012·The International Journal of Biochemistry & Cell Biology·Marina Verkhovskaya, Dmitry A Bloch
Nov 8, 2011·Chemical Communications : Chem Comm·Philip A Ash, Kylie A Vincent
Jul 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elena GorbikovaRuslan Kalendar
Mar 15, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Livia GiottaMassimo Trotta

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