ATRA improves endothelial dysfunction in atherosclerotic rabbits by decreasing CAV‑1 expression and enhancing eNOS activity.

Molecular Medicine Reports
Yan WuHuaqing Zhu

Abstract

The aim of the present study was to explore the protective effects and possible mechanisms of all‑trans‑retinoic acid (ATRA) against atherosclerosis (AS). Rabbits were randomly allocated for standard or high‑fat diet with or without ATRA. After 12 weeks, the aortic rings of the rabbits were removed. Endothelium‑dependent relaxation (EDR) induced by acetylcholine and non‑endothelium‑dependent relaxation induced by sodium nitroprusside in the thoracic aorta were evaluated. NO level and eNOS activity were measured according to the protocol of NO and eNOS ELISA kits. The permeability and morphology of the arterial walls were identified by immunofluorescence and H&E staining respectively. The expression of caveolin‑1 (CAV‑1) and occludin was analyzed using western blotting and immunohistochemistry. The EDR function was significantly reduced in the AS rabbits compared with the normal group, however it was elevated following treatment with ATRA. The eNOS activity and NO level were reduced in the AS group, however were notably increased following oral administration of ATRA. There was an enhancement of endothelial permeability in the AS group compared with the normal group, which decreased following ATRA treatment. Western blot analysi...Continue Reading

References

May 16, 2003·Vascular Pharmacology·Sarah Y Yuan
Oct 29, 2003·Circulation·Subodh VermaTodd J Anderson
Nov 1, 2003·The International Journal of Biochemistry & Cell Biology·Xiao feng YeWen jin Su
Jul 23, 2004·Cardiovascular Ultrasound·Marie-Claude DroletMarie Arsenault
Jul 13, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Anthony ZulliDavid L Hare
Dec 24, 2005·Physiological Reviews·Dolly Mehta, Asrar B Malik
Sep 27, 2006·Handbook of Experimental Pharmacology·M Simionescu, F Antohe
May 4, 2007·Journal of Cellular Physiology·Jenny A G McKenzie, Anne J Ridley
Nov 28, 2007·Annual Review of Pathology·Göran K HanssonCecilia Söderberg-Nauclér
Apr 22, 2008·European Journal of Pharmacology·Ying XuRob H Henning
Sep 18, 2008·Cell and Tissue Research·Anca V SimaMaya Simionescu
Jan 2, 2010·Biochemical and Biophysical Research Communications·Di-xian LuoDuan-fang Liao
Jun 29, 2010·The American Journal of Pathology·Carlos Fernández-HernandoWilliam C Sessa
Jul 27, 2010·Advanced Drug Delivery Reviews·Nathan Bushue, Yu-Jui Yvonne Wan
Aug 4, 2010·Autoimmunity Reviews·S SitiaM Turiel
Oct 12, 2010·Immunopharmacology and Immunotoxicology· SiddikuzzamanV M Berlin Grace

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Citations

Dec 28, 2019·International Journal of Molecular Sciences·Ilja L Kruglikov, Philipp E Scherer
Jan 10, 2020·Oxidative Medicine and Cellular Longevity·Giorgio AquilaPaola Rizzo

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