Atrazine induction of a family 4 cytochrome P450 gene in Chironomus tentans (Diptera: Chironomidae)

Chemosphere
Diana K LondoñoM J Lydy

Abstract

Cytochrome P450-dependent microsomal monooxygenase (P450) activity was measured in control and atrazine-exposed third instar midge larvae, Chironomus tentans. Significantly elevated O-demethylase activity was observed in gut homogenates taken from midges exposed to atrazine concentrations from 1 to 10 ppm for 90 h. No significant induction was observed at atrazine concentrations below 1 ppm. A region of a cytochrome P450 family 4 gene was amplified and sequenced from C. tentans larvae. Alignments of inferred amino acid sequences with other insect CYP4 gene homologues indicate a high degree of similarity. Northern blot analysis employing the CYP4 gene fragment as a probe showed an over-expression in C. tentans exposed to atrazine. The results support the previously identified inducibility of cytochrome P450-dependent activity and provide insight into the potential consequences of atrazine exposure to aquatic organisms.

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Citations

Sep 1, 2006·Archives of Environmental Contamination and Toxicology·M N WacksmanM J Lydy
Nov 2, 2013·Environmental Toxicology and Pharmacology·Prakash M Gopalakrishnan NairJinhee Choi
Jan 22, 2015·International Journal of Molecular Sciences·Xiao LiangKun Yan Zhu
Jul 9, 2010·Chemosphere·Benjamin J ThorntonBlair D Siegfried
Dec 3, 2014·Journal of Insect Physiology·Andrea VogelAnthony C Fiumera
Sep 8, 2010·Environmental Toxicology and Chemistry·Inês DominguesAntónio J A Nogueira
Apr 1, 2006·Chemosphere·Sébastien BoyerPatrick Ravanel
Mar 21, 2015·Environmental Science and Pollution Research International·Elsa BonnaféFlorence Geret
Aug 23, 2006·Expert Opinion on Drug Metabolism & Toxicology·Wladyslawa Anna Daniel
Nov 12, 2013·Toxicology and Industrial Health·Nahla Elsayed Omran, Wesam Mohamed Salama
May 20, 2016·Journal of Pesticide Science·Toshiyuki Katagi, Hitoshi Tanaka
Jan 19, 2005·Pest Management Science·Derek W GammonKeith F Pfeifer

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