Atrial natriuretic peptide modulates cystic fibrosis transmembrane conductance regulator chloride channel expression in rat proximal colon and human intestinal epithelial cells

The Journal of Endocrinology
H J NovairaM M Morales

Abstract

The cystic fibrosis transmembrane conductance regulator (CFTR) is one of the most intensively investigated Cl- channels. Different mutations in the CFTR gene cause the disease cystic fibrosis (CF). CFTR is expressed in the apical membrane of various epithelial cells including the intestine. The major organ affected in CF patients is the lung, but it also causes an important dysfunction of intestinal ion transport. The modulation of CFTR mRNA expression by atrial natriuretic peptide (ANP) was investigated in rat proximal colon and in human intestinal CaCo-2 cells by RNase protection assay and semi-quantitative reverse transcriptase PCR techniques. Groups of rats subjected to volume expansion or intravenous infusion of synthetic ANP showed respective increases of 60 and 50% of CFTR mRNA expression in proximal colon. CFTR mRNA was also increased in cells treated with ANP, reaching a maximum effect at 10(-9) M ANP, probably via cGMP. ANP at 10(-9) M was also able to stimulate both the CFTR promoter region (by luciferase assay) and protein expression in CaCo-2 cells (by Western blot and immunoprecipitation/phosphorylation). These results suggested the involvement of ANP, a hormone involved with extracellular volume, in the expressio...Continue Reading

Citations

Dec 13, 2012·Science Signaling·Sahana Suresh BabuMarco Cattaruzza
Jun 1, 2012·American Journal of Physiology. Cell Physiology·Geoffrey I Sandle, Vazhaikkurichi M Rajendran
Mar 17, 2018·International Journal of Molecular Sciences·Jianjun ChangHong-Long Ji
Apr 19, 2013·American Journal of Physiology. Cell Physiology·Ruhul AminHatim A Hassan
Sep 29, 2006·World Journal of Gastroenterology : WJG·Chun-Hui LiWen-Xie Xu
Nov 26, 2010·Current Opinion in Gastroenterology·Kris A Steinbrecher, Mitchell B Cohen
Mar 1, 2009·Biophysics Reviews·J Souza-Menezes, M M Morales

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