Atrial natriuretic peptide reduces the α-subunit of the epithelial sodium channel mRNA expression in the mouse stria vascularis

Biomedical Reports
Yan LuoYuedi Tang

Abstract

Atrial natriuretic peptide (ANP) is extensively expressed in the cochlea, including the strial vascularis (StV). ANP may participate in the regulation of the water-electrolyte balance. However, the functional significance of ANP in the cochlea is less understood and little is known regarding the exact mechanisms. Studies suggest that the epithelial sodium channel (ENaC) is important for regulating sodium transport across epithelia. ENaC may be involved in the clearance of endolymphatic Na(+) and maintenance of a K-rich and Na-poor composition in the endolymph. Whether ANP has a regulatory effect on the Na(+) channel in the StV remains unknown. The aim of the present study was to evaluate whether ANP affects the expression of the α-subunit of the ENaC (α-ENaC) mRNA in the mouse StV, using the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) technique. The mouse StV tissues were incubated with 10(-6) mol/1 ANP for different times (2, 6, 12, 24 and 48 h) and were subsequently harvested. α-ENaC mRNA was extracted for RT-qPCR analysis of the expression. The study demonstrated the existence of α-ENaC in the mouse StV. Tissues treated with ANP (10(-6) mol/1) showed a significant reduction in α-ENaC mRNA expressio...Continue Reading

References

Jul 1, 1990·Physiological Reviews·B M BrennerM L Zeidel
Jan 1, 1989·Acta Oto-laryngologica. Supplementum·A Meyer zum Gottesberge, J Lamprecht
Jan 1, 1988·Archives of Oto-rhino-laryngology·J Lamprecht, A M Meyer zum Gottesberge
Dec 1, 1983·Canadian Journal of Physiology and Pharmacology·A D BainesH Sonnenberg
Jan 1, 1995·Acta Oto-laryngologica. Supplementum·A Meyer zum GottesbergeR Gerzer
Apr 1, 1997·Physiological Reviews·H Garty, L G Palmer
Jan 30, 1999·Hearing Research·T SeebacherJ E Schultz
Aug 19, 1999·Hearing Research·D C Marcus, T Chiba
Feb 24, 2001·American Journal of Physiology. Renal Physiology·V CouloignerE Ferrary
Dec 4, 2003·Circulation Research·Renate B Pilz, Darren E Casteel
Apr 28, 2005·Current Medicinal Chemistry. Cardiovascular and Hematological Agents·Luis Bolanos Cea
Sep 30, 2005·Journal of the American Society of Nephrology : JASN·Johannes Loffing, Laurent Schild
Aug 30, 2008·Journal of the American Society of Nephrology : JASN·Vivek Bhalla, Kenneth R Hallows

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Citations

Jun 30, 2018·Physiological Genomics·Janet L Fitzakerley, George J Trachte
May 12, 2019·Journal of Molecular and Cellular Cardiology·Ambili MenonDawood Darbar

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