Atropine: a sensitive gas chromatography-mass spectrometry assay and prepharmacokinetic studies

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M Eckert, P H Hinderling

Abstract

A novel assay method for the determination of atropine in biological fluids is presented. Atropine is extracted and subsequently hydrolyzed. The generated tropine is them derivatized to heptafluorobutyryl-tropine, which is measured by GLC-MS. Base peaks m/z 124 and m/z 127 are simultaneously monitored. Deuterated atropine serves as internal standard. This specific and sensitive assay permits a delineation of the disposition pharmacokinetics in humans after i.v. administration of the drug. Prepharmacokinetic studies determined pKa values for atropine and tropine of 9.56 and 10.35 respectively at 22 degrees C. Solvent partitioning experiments showed that atropine exerts a significantly larger lipophilicity than tropine. This was consistent with tropine (0%, 0.85) at 37 degrees C and pH 7.4. Plasma protein binding and erythrocyte partitioning of both compounds were concentration independent and invariable in the presence or absence of each other.

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Citations

Nov 13, 2002·Forensic Science International : Synergy·Akira NameraTohru Kojima
Jul 1, 1985·Journal of Pharmaceutical Sciences·P H HinderlingO Schmidlin
Jan 10, 2002·Journal of Pharmaceutical Sciences·Patrick Poulin, Frank-Peter Theil
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Oct 23, 2012·Forensic Science International : Synergy·Florian RicardJean Claude Alvarez
Jan 17, 2009·Forensic Science International : Synergy·Jochen BeyerHans H Maurer
Feb 8, 2014·Journal of Forensic Sciences·Jérémy CarlierYvan Gaillard
May 10, 1985·Journal of Chromatography·N Kucharczyk, F H Segelman

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