Atropisomeric determination of chiral hydroxylated metabolites of polychlorinated biphenyls using HPLC-MS

Chemistry Central Journal
Guangshu ZhaiJerald L Schnoor

Abstract

Polychlorinated biphenyls (PCBs) are a group of environmental persistent organic pollutants, which can be metabolized into a series of metabolites, including hydroxylated metabolites (OH-PCBs) in biota. Nineteen of 209 PCB congeners can form chiral stable isomers. However, atropisomeric determination of the hydroxylated metabolites of these chiral PCBs has never been reported by LC methods. In this work, a novel HPLC-MS method was developed to detect five chiral OH-PCBs (4OH-PCB91, 5OH-PCB91, 4OH-PCB95, 5OH-PCB95 and 5OH-PCB149) using HPLC-MS without a derivatization step. The influences of column-type, column temperature, flow rate and ratio of the mobile phase on the atropisomeric separation were investigated in detail. In the final method, calibration curves, based on peak areas against concentration, were linear in a range of 1-100 ng mL-1 of five chiral OH-PCBs with correlation coefficients ranging from 0.9996 to 0.9999 for all atropisomers of OH-PCBs. The relative standard deviations measured at the 10.0 ng mL-1 level for atropisomers of five chiral OH-PCBs were in the range of 0.60-7.55% (n = 5). Calculated detection limits (S/N = 3) of five chiral OH-PCBs were between 0.31 and 0.60 ng mL-1 for all OH-PCB atropisomers. T...Continue Reading

References

Jan 1, 1991·Archives of Environmental Contamination and Toxicology·M S EvansC P Rice
Jan 1, 1995·Chemical Research in Toxicology·U BauerL W Robertson
May 1, 1994·Environmental Health Perspectives·A BergmanH Kuroki
Jan 12, 2000·Archives of Environmental Contamination and Toxicology·R SerranoM J Gonzalez
May 16, 2001·Environmental Science & Technology·C S WongW T Foreman
Jul 4, 2001·Environmental Science & Technology·C S WongW T Foreman
Nov 7, 2001·Chemosphere·H J Lehmler, L W Robertson
Nov 14, 2001·Archives of Environmental Contamination and Toxicology·L HovanderE Klasson Wehler
May 10, 2002·Environmental Science & Technology·Paul F HoekstraDerek C G Muir
Sep 3, 2002·Environmental Health Perspectives·Britta FängströmAke Bergman
Apr 13, 2004·Environmental Science & Technology·Matthew Robson, Stuart Harrad
Mar 21, 2007·Environmental Health Perspectives·June-Soo ParkIrva Hertz-Picciotto
Sep 2, 2008·Journal of Chromatography. a·Izabela Kania-KorwelHans-Joachim Lehmler
Oct 23, 2008·Environmental Science & Technology·Matthew S RossCharles S Wong
Dec 11, 2008·Applied Microbiology and Biotechnology·Satoru FujihiroShigenori Sonoki
Apr 14, 2010·Environmental Science & Technology·Hans-Joachim LehmlerCharles S Wong
Sep 15, 2012·Environmental Science & Technology·Izabela Kania-KorwelHans-Joachim Lehmler

❮ Previous
Next ❯

Citations

Jan 30, 2015·Critical Reviews in Toxicology·Fabian A GrimmLarry W Robertson
Dec 18, 2015·Environmental Science and Pollution Research International·Cunxian MaJerald L Schnoor
Feb 6, 2015·Environmental Science and Pollution Research International·Izabela Kania-Korwel, Hans-Joachim Lehmler
Feb 17, 2018·Chirality·Xiaolei ChenSu Zeng
Jul 27, 2017·Environmental Science and Pollution Research International·Kiran DhakalLarry W Robertson
Dec 6, 2017·Journal of Chromatographic Science·Yongqiang LiuYing An

❮ Previous
Next ❯

Software Mentioned

AccuStandard

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.