ATRQβ-001 vaccine prevents atherosclerosis in apolipoprotein E-null mice

Journal of Hypertension
Yanzhao ZhouXiao Chen

Abstract

Angiotensin II (AngII) type 1 receptor (AT1R) blockers have been proved to reduce atherosclerosis. Previously, we have invented ATRQβ-001 vaccine which showed a desirable blocking effect for AT1R. The purpose of this study was to investigate whether ATRQβ-001 vaccine would prevent atherosclerosis in apolipoprotein E-null (ApoE-/-) mice. Male ApoE-/- mice were administered with ATRQβ-001 vaccine, Qβ virus-like particles, valsartan or vehicle over a period of 24 weeks. In vitro, human coronary artery endothelial cells preincubated with the anti-ATR-001 antibody, the neutralization antibody or valsartan for 2  h, were treated with AngII for 24  h. Histological stain and molecule biology methods were used to assess the atheroprotective effect of the vaccine. ATRQβ-001 vaccine significantly reduced the lesion area and promoted the stability of atherosclerotic plaque. Meanwhile, macrophage infiltration as well as the expressions of adhesion molecules and monocyte chemoattractant protein-1 was obviously decreased in the ATRQβ-001 vaccine group. Additionally, the vaccine markedly reduced the apoptosis in the lesions of the ApoE-/- mice. In vitro, the anti-ATR-001 antibody inhibited endothelial apoptosis induced by AngII. Furthermore, A...Continue Reading

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Citations

Nov 8, 2016·Expert Review of Vaccines·Dania O Govea-AlonsoSergio Rosales-Mendoza
Oct 4, 2017·Scientific Reports·Yajie PanYuhua Liao
Dec 28, 2018·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Yajie PanZhihua Qiu
Sep 6, 2019·International Journal of Hypertension·Tatsuhiko Azegami, Hiroshi Itoh
Oct 1, 2020·Heart Failure Reviews·Noé Francisco Garay-GutiérrezCarlos Enrique Guerrero-Beltrán
Sep 13, 2019·Journal of the American Heart Association·Hongrong ZhangXiao Chen
Oct 5, 2017·Scientific Reports·Xiajun HuYuhua Liao
Feb 1, 2021·Biochemical and Biophysical Research Communications·Yingxuan WangXiao Chen

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Methods Mentioned

BETA
protein assay
ELISA
Assay
dissection
Flow cytometry
light microscopy

Software Mentioned

GraphPad
Pro Plus
Image
Prism
FlowJo

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