ATRX in Diffuse Gliomas With its Mosaic/Heterogeneous Expression in a Subset.

Brain Pathology
Suvendu PurkaitChitra Sarkar

Abstract

This study aims (1) to evaluate ATRX expression in different grades and subtypes of gliomas and correlate with other hallmark genetic alterations, (2) to identify and characterize mosaic/heterogeneous staining in gliomas in terms of mutation status. One hundred seventy six cases of glioma were assessed for ATRX immunohistochemistry and subdivided into positive, negative and mosaic/heterogeneous staining patterns. Five cases with heterogeneous staining were further subjected to next generation sequencing. Higher frequency of ATRX immune-negativity was detected in grade II/III astrocytic, oligoastrocytic tumors and secondary glioblastomas (GBMs), while infrequent in primary GBMs and rare in oligodendrogliomas. Loss of expression was significantly associated with IDH1 and/or TP53 mutation, while mutually exclusive with 1p/19q codeletion. Mosaic/heterogeneous staining was detected exclusively in GBMs (21.2%). Two different types of mosaic staining were identified (1) Admixture of positive and negative nuclei or intermixed mosaic and (2) Separate fragments with positive and negative/intermixed mosaic staining. ATRX mutation was identified in 2/5 (40%) cases with mosaic staining while one case showed DAXX mutation. All these cases we...Continue Reading

References

Apr 26, 2007·The American Journal of Pathology·Hiroko Ohgaki, Paul Kleihues
Jan 30, 2008·The Journal of Cell Biology·Kieran RitchieNathalie G Bérubé
May 8, 2008·PloS One·Andrew R BassettAndrew A Travers
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Feb 16, 2010·The Journal of Biological Chemistry·Alexander V EmelyanovDmitry V Fyodorov
Jul 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Peter W LewisC David Allis
Jun 15, 2011·Nature Structural & Molecular Biology·Sebastian EustermannDavid Neuhaus
Jul 2, 2011·Science·Christopher M HeaphyAlan K Meeker
Nov 18, 2011·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Prerana JhaChitra Sarkar
Mar 15, 2012·JAMA : the Journal of the American Medical Association·Nai-Kong V CheungUNKNOWN St Jude Children's Research Hospital–Washington University Pediatric Cancer Genome Project
Oct 19, 2012·Nature·Simon J ElsässerDinshaw J Patel
Jun 15, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Malak AbedalthagafiArie Perry
Jul 6, 2014·Brain Pathology·David N LouisUNKNOWN International Society Of Neuropathology--Haarlem
Jun 11, 2015·The New England Journal of Medicine·UNKNOWN Cancer Genome Atlas Research NetworkJianan Zhang

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Citations

Mar 20, 2018·Brain Tumor Pathology·Akane YamamichiAtsushi Natsume
Oct 17, 2017·Frontiers in Oncology·Pravanya NandakumarSunit Das
Jun 15, 2020·Cellular and Molecular Neurobiology·José Alberto Carlos-EscalanteTalia Wegman-Ostrosky
Feb 19, 2021·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Jingjing YaoYueshan Piao

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