Attached and unattached bacterial communities in a 120-meter corehole in an acidic, crystalline rock aquifer

Applied and Environmental Microbiology
R M LehmanF S Colwell

Abstract

The bacteria colonizing geologic core sections (attached) were contrasted with those found suspended in the groundwater (unattached) by examining the microbiology of 16 depth-paired core and groundwater samples using a suite of culture-independent and culture-dependent analyses. One hundred twenty-two meters was continuously cored from a buried chalcopyrite ore hosted in a biotite-quartz-monzonite porphyry at the Mineral Park Mine near Kingman, Ariz. Every fourth 1.5-m core was acquired using microbiologically defensible methods, and these core sections were aseptically processed for characterization of the attached bacteria. Groundwater samples containing unattached bacteria were collected from the uncased corehole at depth intervals corresponding to the individual cores using an inflatable straddle packer sampler. The groundwater was acidic (pH 2.8 to 5.0), with low levels of dissolved oxygen and high concentrations of sulfate and metals, including ferrous iron. Total numbers of attached cells were less than 10(5) cells g of core material(-1) while unattached cells numbered about 10(5) cells ml of groundwater(-1). Attached and unattached acidophilic heterotrophs were observed throughout the depth profile. In contrast, acidoph...Continue Reading

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Citations

Nov 6, 2007·Applied and Environmental Microbiology·Jason W SahlJohn R Spear
Jul 4, 2003·Applied and Environmental Microbiology·Brian J Mailloux, Mark E Fuller
Jan 11, 2005·Applied and Environmental Microbiology·Jutta KleikemperJosef Zeyer
Oct 6, 2005·Applied and Environmental Microbiology·Helen A VrionisDerek R Lovley
Dec 8, 2005·Applied and Environmental Microbiology·Jessica SimbahanPaul Blum
Aug 13, 2013·PloS One·James P R SorensenPeter J Williams
May 26, 2010·Ground Water·J KozuskanichB C Anderson
Aug 14, 2013·Ground Water·Fabio UgoliniMartin H Schroth
Sep 12, 2009·FEMS Microbiology Ecology·Krista LongneckerElizabeth B Kujawinski
Aug 11, 2016·FEMS Microbiology Ecology·Paula J MouserKelly C Wrighton
Dec 21, 2005·Research in Microbiology·Tetyana LytvynenkoNatalia Kozyrovska
Nov 26, 2010·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Jin-Hoon KimYoung Kim
Jul 6, 2013·MicrobiologyOpen·Inés C WilhartitzAndreas H Farnleitner
Aug 4, 2018·Frontiers in Microbiology·Rose M JonesBeth N Orcutt
Mar 2, 2021·Environmental Pollution·Juan F Mujica-AlarconStephen A Rolfe
May 24, 2008·Environmental Science & Technology·Yoshiko FujitaRobert W Smith

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