Attachment and growth of human keratinocytes in a serum-free environment

Journal of Cellular Physiology
B A GilchrestT Maciag

Abstract

Using a serum-free system, we have investigated the influence of human fibronectin (HFN) and selected growth factors (GF) on the attachment and growth of normal human keratinocytes in vitro. Single-cell suspensions of keratinocytes from near-confluent primary plates, plated on 5-10 microgram/cm2 HFN, showed approximately 30-40% attachment after 2-24 hours of incubation at 37 degrees C, compared with 4-6% attachment on uncoated platic plates. Percentage of attached cells was independent of seed density, tissue donor age, in vitro culture age, or medium composition, while subsequent cellular proliferation was strongly dependent on these factors. Keratinocytes grown on an adequate HFN matrix in a previously described hormone-supplemented medium (Maciag et al., 1981a) achieved four to eight population doubling over 7-12 days at densities greater than or equal to 104 cell/cm2. Removal of most GF individually from the medium had little or no effect on growth, while removal of epidermal growth factor (EGF) alone reduced growth by 30-35% and removal of bovine brain extract (BE) alone reduced growth by approximately 90%. Conversely, EGF alone in basal medium supported approximately 10% control growth, BE alone supported 30-40% control g...Continue Reading

References

Aug 1, 1979·Journal of Cellular Physiology·T JenningsA Lipton
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F C WestallD Gospodarowicz
Apr 1, 1979·Experimental Cell Research·C L Marcelo
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T MaciagR Forand
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J HavrankovaM Brownstein
Jan 1, 1979·The Journal of Cell Biology·J C MurrayS I Katz
May 1, 1979·The Journal of Investigative Dermatology·B A Gilchrest
Aug 1, 1978·The Journal of Investigative Dermatology·S C Liu, M Karasek
Apr 1, 1977·Experimental Cell Research·L B ChenJ K McDougall
Jul 15, 1977·International Journal of Cancer. Journal International Du Cancer·E Engvall, E Ruoslahti
Apr 12, 1972·Nature: New Biology·J L ClarkG H Sato
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D GospodarowiczG Sato
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D Gospodarowicz, J S Moran
May 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R W Holley, J A Kiernan
Nov 1, 1980·Cell Biology International Reports·B A GilchrestT Maciag
Jan 1, 1981·Differentiation; Research in Biological Diversity·D BarritaultY Courtois
Mar 1, 1981·The Journal of Cell Biology·H K KleinmanG R Martin
Nov 1, 1981·The Journal of Cell Biology·T MaciagR Weinstein
Oct 9, 1980·The New England Journal of Medicine·M B Sporn, G J Todaro
Dec 1, 1980·Cell·D Barnes, G Sato

❮ Previous
Next ❯

Citations

Mar 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·F L VaughanI A Bernstein
Aug 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M PonecJ Boonstra
Jun 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M Reiss, C L Dibble
Jan 1, 1993·Tissue & Cell·J P DenèfleJ P Lechaire
Jul 1, 1986·Mechanisms of Ageing and Development·B M Stanulis-Praeger, B A Gilchrest
Apr 1, 1993·Burns : Journal of the International Society for Burn Injuries·L GermainF A Auger
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Taub
Oct 20, 2005·Journal of Bioscience and Bioengineering·M Kino-OkaJ E Prenosil
Jul 1, 1985·The Journal of Cell Biology·D J DonaldsonL T Furcht
Dec 1, 1987·The Journal of Cell Biology·K TodaF Grinnell
Nov 1, 1983·The Journal of Cell Biology·T NishidaR Manabe
Jan 1, 1989·Journal of Ocular Pharmacology·M M Tahery, D A Lee
Aug 1, 1993·The Journal of Dermatologic Surgery and Oncology·T J Phillips
Feb 1, 1985·Journal of the American Academy of Dermatology·M Eisinger
Apr 25, 2006·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Markus M ZimmerHans Becker
Jul 1, 1984·Clinics in Dermatology·B A Gilchrest
Jan 1, 1994·Clinics in Dermatology·T J Phillips
Jan 1, 1994·Clinics in Dermatology·M L Cooper, R L Spielvogel
Aug 1, 1986·The British Journal of Dermatology·B A Gilchrest
Jun 1, 1988·International Journal of Dermatology·J Fransson, H Hammar
Apr 1, 1989·The British Journal of Dermatology·R R IsseroffR M Rosenberg
Apr 1, 1996·International Journal of Oral and Maxillofacial Surgery·M FormanekJ Kornfehl
Mar 25, 2014·Journal of Biomedical Materials Research. Part a·Isa P MonteiroPaula T Hammond
Nov 1, 1983·Journal of Cellular Physiology·B A GilchrestT Maciag
Sep 1, 1985·Journal of Cellular Physiology·E J O'KeefeN Russell
Jun 1, 1988·Journal of Cellular Physiology·P R GordonB A Gilchrest
Sep 1, 1984·Journal of Cellular Physiology·B A GilchrestT Maciag
May 3, 2014·Macromolecular Bioscience·Monica Suryana TjinEileen Fong
Feb 15, 1984·International Journal of Cancer. Journal International Du Cancer·K W Brown, E K Parkinson
Aug 26, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·B PomahacE Eriksson
Jan 1, 1990·The Annals of Otology, Rhinology, and Laryngology·J L KasperbauerR E Scott
Jan 1, 1984·Journal of Cellular Biochemistry·F Grinnell
May 1, 1991·The Journal of Investigative Dermatology·M PartridgeM Feldmann
Nov 15, 1986·Thrombosis Research·K Igisu
Feb 1, 1984·The Journal of Investigative Dermatology·E J O'KeefeR E Payne
Nov 1, 1984·The Journal of Investigative Dermatology·A Takashima, F Grinnell
Dec 1, 1984·The Journal of Investigative Dermatology·K S Stenn, L M Milstone
Jun 1, 1984·The Journal of Investigative Dermatology·M KuboR A Clark
Nov 1, 1987·The Journal of Investigative Dermatology·F FurukawaD A Norris
Jul 1, 1985·The Journal of Investigative Dermatology·D N Sauder
Aug 1, 1985·The Journal of Investigative Dermatology·E J O'KeefeD T Woodley

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